8QUA

GTP binding protein YsxC from Staphylococcus aureus

8QUA の概要

• エントリーDOI: 10.2210/pdb8qua/pdb
• 分子名称: Probable GTP-binding protein EngB, ACETYL GROUP, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: ysxc, ribosome, gtpase, protein binding
• 由来する生物種: Staphylococcus aureus subsp. aureus N315
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22849.11

構造登録者

Biktimirov, A.,Islamov, D.,Lazarenko, V.,Fatkhullin, B.,Validov, S.,Yusupov, M.,Usachev, K. (登録日: 2023-10-15, 公開日: 2024-02-07)

主引用文献

Biktimirov, A.,Islamov, D.,Fatkhullin, B.,Lazarenko, V.,Validov, S.,Yusupov, M.,Usachev, K.
Crystal structure of GTPase YsxC from Staphylococcus aureus.
Biochem.Biophys.Res.Commun., 699:149545-149545, 2024

PubMed Abstract: The YsxC protein from Staphylococcus aureus is a GTP-binding protein from the TRAFAC superfamily of the TrmE-Era-EngA-EngB-Septin-like GTPase class, EngB family of GTPases. Recent structural and biochemical studies of YsxC function show that it is an integral part of the pathogenic microorganism life cycle, as it is involved in the assembly of the large 50S ribosomal subunit. Structural studies of this protein with its specific functional features make it an attractive target for further development of new selective antimicrobials. In this study, we cloned the ysxC protein gene from S. aureus, overexpressed the protein in E. coli, and subsequently purified and crystallized it. Protein crystals were successfully grown using the vapor diffusion method, yielding diffraction data with a resolution of up to 2 Å. Comparative analysis of the structure of SaYsxC with known three-dimensional structures of homologs from other microorganisms showed the presence of structural differences for the apo form.

PubMed: 38277729
DOI: 10.1016/j.bbrc.2024.149545

実験手法

X-RAY DIFFRACTION (2 Å)