8QSZ

Structure of s. pombe RNA polymerase II in complex with DSIF and Rat1/Rai1

8QSZ の概要

• エントリーDOI: 10.2210/pdb8qsz/pdb
• EMDBエントリー: 18643
• 分子名称: DNA-directed RNA polymerase II subunit rpb1, DNA-directed RNA polymerase II subunit RPB11, DNA-directed RNA polymerases I, II, and III subunit RPABC4, ... (17 entities in total)
• 機能のキーワード: rna polymerase ii, rai1, rat1, spt5, dsif, termination, exoribonuclease, rna binding protein
• 由来する生物種: Schizosaccharomyces pombe (fission yeast); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 748771.05

構造登録者

Carrique, L.,Kus, K.,Vasiljeva, L.,Grimes, J.M. (登録日: 2023-10-12, 公開日: 2024-10-23, 最終更新日: 2025-01-22)

主引用文献

Kus, K.,Carrique, L.,Kecman, T.,Fournier, M.,Hassanein, S.S.,Aydin, E.,Kilchert, C.,Grimes, J.M.,Vasiljeva, L.
DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts.
Nat Commun, 16:10-10, 2025

PubMed Abstract: Precursor messenger RNA (pre-mRNA) is processed into its functional form during RNA polymerase II (Pol II) transcription. Although functional coupling between transcription and pre-mRNA processing is established, the underlying mechanisms are not fully understood. We show that the key transcription termination factor, RNA exonuclease Xrn2 engages with Pol II forming a stable complex. Xrn2 activity is stimulated by Spt5 to ensure efficient degradation of nascent RNA leading to Pol II dislodgement from DNA. Our results support a model where Xrn2 first forms a stable complex with the elongating Pol II to achieve its full activity in degrading nascent RNA revising the current 'torpedo' model of termination, which posits that RNA degradation precedes Xrn2 engagement with Pol II. Spt5 is also a key factor that attenuates the expression of non-coding transcripts, coordinates pre-mRNA splicing and 3'-end processing. Our findings indicate that engagement with the transcribing Pol II is an essential regulatory step modulating the activity of RNA enzymes such as Xrn2, thus advancing our understanding of how RNA maturation is controlled during transcription.

PubMed: 39746995
DOI: 10.1038/s41467-024-55063-7

実験手法

ELECTRON MICROSCOPY (2.67 Å)