8QQC

Crystal structure of Lymantria dispar CPV14 polyhedra single crystal

8QQC の概要

• エントリーDOI: 10.2210/pdb8qqc/pdb
• 分子名称: Polyhedrin, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: polyhedrin, gtp binding, viral protein
• 由来する生物種: Lymantria dispar (gypsy moth)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29270.93

構造登録者

Trincao, J.,Warren, A.,Crawshaw, A.,Sutton, G.,Stuart, D.,Evans, G. (登録日: 2023-10-04, 公開日: 2023-11-08, 最終更新日: 2024-11-20)

主引用文献

Warren, A.J.,Trincao, J.,Crawshaw, A.D.,Beale, E.V.,Duller, G.,Stallwood, A.,Lunnon, M.,Littlewood, R.,Prescott, A.,Foster, A.,Smith, N.,Rehm, G.,Gayadeen, S.,Bloomer, C.,Alianelli, L.,Laundy, D.,Sutter, J.,Cahill, L.,Evans, G.
VMXm - A sub-micron focus macromolecular crystallography beamline at Diamond Light Source.
J.Synchrotron Radiat., 31:1593-1608, 2024

PubMed Abstract: VMXm joins the suite of operational macromolecular crystallography beamlines at Diamond Light Source. It has been designed to optimize rotation data collections from protein crystals less than 10 µm and down to below 1 µm in size. The beamline has a fully focused beam of 0.3 × 2.3 µm (vertical × horizontal) with a tuneable energy range (6-28 keV) and high flux (1.6 × 10 photons s at 12.5 keV). The crystals are housed within a vacuum chamber to minimize background scatter from air. Crystals are plunge-cooled on cryo-electron microscopy grids, allowing much of the liquid surrounding the crystals to be removed. These factors improve the signal-to-noise during data collection and the lifetime of the microcrystals can be prolonged by exploiting photoelectron escape. A novel in vacuo sample environment has been designed which also houses a scanning electron microscope to aid with sample visualization. This combination of features at VMXm allows measurements at the physical limits of X-ray crystallography on biomacromolecules to be explored and exploited.

PubMed: 39475835
DOI: 10.1107/S1600577524009160

実験手法

X-RAY DIFFRACTION (1.3 Å)