8QOY

Capsular polysaccharide synthesis multienzyme of Actinobacillus Pleuropneumoniae serotype 3

8QOY の概要

• エントリーDOI: 10.2210/pdb8qoy/pdb
• 分子名称: TagF-like capsule polymerase Cps3D, SULFATE ION, ZINC ION, ... (4 entities in total)
• 機能のキーワード: bacterial protein, biosynthetic protein
• 由来する生物種: Actinobacillus pleuropneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 134393.24

構造登録者

Di Domenico, V.,Litschko, C.,Schulze, J.,Bethe, A.,Cifuente, J.O.,Marina, A.,Budde, I.,Mast, T.A.,Sulewska, M.,Berger, M.,Buettner, F.,Gerardy-Schahn, R.,Fiebig, T.,Guerin, M.E. (登録日: 2023-09-29, 公開日: 2024-07-03, 最終更新日: 2025-01-01)

主引用文献

Litschko, C.,Di Domenico, V.,Schulze, J.,Li, S.,Ovchinnikova, O.G.,Voskuilen, T.,Bethe, A.,Cifuente, J.O.,Marina, A.,Budde, I.,Mast, T.A.,Sulewska, M.,Berger, M.,Buettner, F.F.R.,Lowary, T.L.,Whitfield, C.,Codee, J.D.C.,Schubert, M.,Guerin, M.E.,Fiebig, T.
Transition transferases prime bacterial capsule polymerization.
Nat.Chem.Biol., 21:120-130, 2025

PubMed Abstract: Capsules are long-chain carbohydrate polymers that envelop the surfaces of many bacteria, protecting them from host immune responses. Capsule biosynthesis enzymes are potential drug targets and valuable biotechnological tools for generating vaccine antigens. Despite their importance, it remains unknown how structurally variable capsule polymers of Gram-negative pathogens are linked to the conserved glycolipid anchoring these virulence factors to the bacterial membrane. Using Actinobacillus pleuropneumoniae as an example, we demonstrate that CpsA and CpsC generate a poly(glycerol-3-phosphate) linker to connect the glycolipid with capsules containing poly(galactosylglycerol-phosphate) backbones. We reconstruct the entire capsule biosynthesis pathway in A. pleuropneumoniae serotypes 3 and 7, solve the X-ray crystal structure of the capsule polymerase CpsD, identify its tetratricopeptide repeat domain as essential for elongating poly(glycerol-3-phosphate) and show that CpsA and CpsC stimulate CpsD to produce longer polymers. We identify the CpsA and CpsC product as a wall teichoic acid homolog, demonstrating similarity between the biosynthesis of Gram-positive wall teichoic acid and Gram-negative capsules.

PubMed: 38951648
DOI: 10.1038/s41589-024-01664-8

実験手法

X-RAY DIFFRACTION (3 Å)