8QMW
Non-obligately L8S8-complex forming RubisCO derived from ancestral sequence reconstruction and rational engineering in L8S8 complex with substitutions R269W, E271R, L273N
Summary for 8QMW
| Entry DOI | 10.2210/pdb8qmw/pdb |
| Descriptor | RubisCO large subunit, RubisCO small subunit, 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE, ... (6 entities in total) |
| Functional Keywords | rubisco, cabp, ancestral, lyase |
| Biological source | synthetic construct More |
| Total number of polymer chains | 8 |
| Total formula weight | 256346.85 |
| Authors | Zarzycki, J.,Schulz, L.,Erb, T.J.,Hochberg, G.K.A. (deposition date: 2023-09-25, release date: 2024-10-02, Last modification date: 2025-01-15) |
| Primary citation | Schulz, L.,Zarzycki, J.,Steinchen, W.,Hochberg, G.K.A.,Erb, T.J. Layered entrenchment maintains essentiality in the evolution of Form I Rubisco complexes. Embo J., 44:269-280, 2025 Cited by PubMed Abstract: Protein complexes composed of strictly essential subunits are abundant in nature and often arise through the gradual complexification of ancestral precursor proteins. Essentiality can arise through the accumulation of changes that are tolerated in the complex state but would be deleterious for the standalone complex components. While this theoretical framework to explain how essentiality arises has been proposed long ago, it is unclear which factors cause essentiality to persist over evolutionary timescales. In this work we show that the central enzyme of photosynthesis, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), can easily start to depend on a newly recruited interaction partner through multiple, genetically distinct mechanisms that affect stability, solubility, and catalysis. We demonstrate that layering multiple mechanisms of essentiality can lead to its persistence, even if any given mechanism reverts. More broadly, our work highlights that new interaction partners can drastically re-shape which substitutions are tolerated in the proteins they are recruited into. This can lead to the evolution of multilayered essentiality through the exploration of areas of sequence space that are only accessible in the complex state. PubMed: 39558108DOI: 10.1038/s44318-024-00311-1 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.75 Å) |
Structure validation
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