8QLL

Crystal structure of rat glutathione transferase Omega 1 bound to glutathione

8QLL の概要

• エントリーDOI: 10.2210/pdb8qll/pdb
• 分子名称: Glutathione S-transferase omega-1, GLUTATHIONE (3 entities in total)
• 機能のキーワード: glutathione transferase omega, thiol-transferase, ligandin, transferase
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 57682.29

構造登録者

Schwartz, M.,Neiers, F. (登録日: 2023-09-20, 公開日: 2024-07-31)

主引用文献

Poirier, N.,Menetrier, F.,Moreno, J.,Boichot, V.,Heydel, J.M.,Didierjean, C.,Canivenc-Lavier, M.C.,Canon, F.,Neiers, F.,Schwartz, M.
Rattus norvegicus Glutathione Transferase Omega 1 Localization in Oral Tissues and Interactions with Food Phytochemicals.
J.Agric.Food Chem., 72:5887-5897, 2024

PubMed Abstract: Glutathione transferases are xenobiotic-metabolizing enzymes with both glutathione-conjugation and ligandin roles. GSTs are present in chemosensory tissues and fluids of the nasal/oral cavities where they protect tissues from exogenous compounds, including food molecules. In the present study, we explored the presence of the omega-class glutathione transferase (GSTO1) in the rat oral cavity. Using immunohistochemistry, GSTO1 expression was found in taste bud cells of the tongue epithelium and buccal cells of the oral epithelium. Buccal and lingual extracts exhibited thiol-transferase activity (4.9 ± 0.1 and 1.8 ± 0.1 μM/s/mg, respectively). A slight reduction from 4.9 ± 0.1 to 4.2 ± 0.1 μM/s/mg ( < 0.05; Student's test) was observed in the buccal extract with 100 μM GSTO1-IN-1, a specific inhibitor of GSTO1. RnGSTO1 exhibited the usual activities of omega GSTs, .., thiol-transferase (catalytic efficiency of 8.9 × 10 M·s), and phenacyl-glutathione reductase (catalytic efficiency of 8.9 × 10 M·s) activities, similar to human GSTO1. RnGSTO1 interacts with food phytochemicals, including bitter compounds such as luteolin (K = 3.3 ± 1.9 μM). Crystal structure analysis suggests that luteolin most probably binds to RnGSTO1 ligandin site. Our results suggest that GSTO1 could interact with food phytochemicals in the oral cavity.

PubMed: 38441878
DOI: 10.1021/acs.jafc.4c00483

実験手法

X-RAY DIFFRACTION (2.4 Å)