8QL0

Structure of human PAD6 Phosphomimic mutant V10E/S446E, apo

8QL0 の概要

• エントリーDOI: 10.2210/pdb8ql0/pdb
• 分子名称: Protein-arginine deiminase type-6, DI(HYDROXYETHYL)ETHER, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: peptidylarginine deiminase, apo, mammalian fertilization, cytoplasmic lattice, unknown function
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 78549.33

構造登録者

Ranaivoson, F.M.,Beaumont, E. (登録日: 2023-09-18, 公開日: 2024-05-08, 最終更新日: 2024-05-15)

主引用文献

Ranaivoson, F.M.,Bande, R.,Cardaun, I.,De Riso, A.,Gartner, A.,Loke, P.,Reinisch, C.,Vogirala, P.,Beaumont, E.
Crystal structure of human peptidylarginine deiminase type VI (PAD6) provides insights into its inactivity.
Iucrj, 11:395-404, 2024

PubMed Abstract: Human peptidylarginine deiminase isoform VI (PAD6), which is predominantly limited to cytoplasmic lattices in the mammalian oocytes in ovarian tissue, is essential for female fertility. It belongs to the peptidylarginine deiminase (PAD) enzyme family that catalyzes the conversion of arginine residues to citrulline in proteins. In contrast to other members of the family, recombinant PAD6 was previously found to be catalytically inactive. We sought to provide structural insight into the human homologue to shed light on this observation. We report here the first crystal structure of PAD6, determined at 1.7 Å resolution. PAD6 follows the same domain organization as other structurally known PAD isoenzymes. Further structural analysis and size-exclusion chromatography show that PAD6 behaves as a homodimer similar to PAD4. Differential scanning fluorimetry suggests that PAD6 does not coordinate Ca which agrees with acidic residues found to coordinate Ca in other PAD homologs not being conserved in PAD6. The crystal structure of PAD6 shows similarities with the inactive state of apo PAD2, in which the active site conformation is unsuitable for catalytic citrullination. The putative active site of PAD6 adopts a non-productive conformation that would not allow protein-substrate binding due to steric hindrance with rigid secondary structure elements. This observation is further supported by the lack of activity on the histone H3 and cytokeratin 5 substrates. These findings suggest a different mechanism for enzymatic activation compared with other PADs; alternatively, PAD6 may exert a non-enzymatic function in the cytoplasmic lattice of oocytes and early embryos.

PubMed: 38656308
DOI: 10.1107/S2052252524002549

実験手法

X-RAY DIFFRACTION (1.675 Å)