8QH0

Crystal structure of the SARS-CoV-2 RBD with the antibody Cv2.3194

Summary for 8QH0

• Entry DOI: 10.2210/pdb8qh0/pdb
• Descriptor: Spike protein S1, Cv2.3194 Heavy chain, IGK@ protein, ... (7 entities in total)
• Functional Keywords: coronavirus neutralizing antibody, viral protein
• Biological source: Severe acute respiratory syndrome coronavirus 2; More
• Total number of polymer chains: 3
• Total formula weight: 70993.04

Authors

Fernandez, I.,Rey, F.A. (deposition date: 2023-09-06, release date: 2024-06-19, Last modification date: 2025-07-02)

Primary citation

Planchais, C.,Fernandez, I.,Chalopin, B.,Bruel, T.,Rosenbaum, P.,Beretta, M.,Dimitrov, J.D.,Conquet, L.,Donati, F.,Prot, M.,Porrot, F.,Planas, D.,Staropoli, I.,Guivel-Benhassine, F.,Baquero, E.,van der Werf, S.,Haouz, A.,Simon-Loriere, E.,Montagutelli, X.,Maillere, B.,Rey, F.A.,Guardado-Calvo, P.,Nozach, H.,Schwartz, O.,Mouquet, H.
Broad sarbecovirus neutralization by combined memory B cell antibodies to ancestral SARS-CoV-2.
Iscience, 27:110354-110354, 2024

PubMed Abstract: Antibodies play a pivotal role in protecting from SARS-CoV-2 infection, but their efficacy is challenged by the continuous emergence of viral variants. In this study, we describe two broadly neutralizing antibodies cloned from the memory B cells of a single convalescent individual after infection with ancestral SARS-CoV-2. Cv2.3194, a resilient class 1 anti-RBD antibody, remains active against Omicron sub-variants up to BA.2.86. Cv2.3132, a near pan-Sarbecovirus neutralizer, targets the heptad repeat 2 membrane proximal region. When combined, Cv2.3194 and Cv2.3132 form a complementary SARS-CoV-2 neutralizing antibody cocktail exhibiting a local dose-dependent synergy. Thus, remarkably robust neutralizing memory B cell antibodies elicited in response to ancestral SARS-CoV-2 infection can withstand viral evolution and immune escape. The cooperative effect of such antibody combination may confer a certain level of protection against the latest SARS-CoV-2 variants.

PubMed: 39071888
DOI: 10.1016/j.isci.2024.110354

Experimental method

X-RAY DIFFRACTION (1.87 Å)