8QBD

Helical reconstruction of yeast eisosome protein Pil1 bound to membrane composed of lipid mixture +PIP2/+sterol (DOPC, DOPE, DOPS, cholesterol, PI(4,5)P2 35:20:20:15:10)

Summary for 8QBD

• Entry DOI: 10.2210/pdb8qbd/pdb
• EMDB information: 18310
• Descriptor: Sphingolipid long chain base-responsive protein PIL1, D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE, O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine (3 entities in total)
• Functional Keywords: bar domain, lipid reconstitution, membrane microdomain, lipid binding protein
• Biological source: Saccharomyces cerevisiae (brewer's yeast)
• Total number of polymer chains: 14
• Total formula weight: 554473.00

Authors

Kefauver, J.M.,Zou, L.,Desfosses, A.,Loewith, R.J. (deposition date: 2023-08-24, release date: 2024-07-24)

• Primary citation: Kefauver, J.M.,Hakala, M.,Zou, L.,Alba, J.,Espadas Moreno, J.,Tettamanti, M.G.,Estrozi, L.,Vanni, S.,Roux, A.,Desfosses, A.,Loewith, R.; CryoEM architecture of a native stretch-sensitive membrane microdomain; To Be Published, ; DOI: 10.1038/s41586-024-07720-6

Experimental method

ELECTRON MICROSCOPY (3.61 Å)