8Q91

Structure of the human 20S U5 snRNP core

8Q91 の概要

• エントリーDOI: 10.2210/pdb8q91/pdb
• EMDBエントリー: 18267
• 分子名称: CD2 antigen cytoplasmic tail-binding protein 2, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, ... (15 entities in total)
• 機能のキーワード: snrnp, cd2bp2, spliceosome, u5, splicing
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 1001205.95

構造登録者

Schneider, S.,Galej, W.P. (登録日: 2023-08-19, 公開日: 2024-03-27, 最終更新日: 2025-07-09)

主引用文献

Schneider, S.,Brandina, I.,Peter, D.,Lagad, S.,Fraudeau, A.,Portell-Montserrat, J.,Tholen, J.,Zhao, J.,Galej, W.P.
Structure of the human 20S U5 snRNP.
Nat.Struct.Mol.Biol., 31:752-756, 2024

PubMed Abstract: The 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA-protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. CD2BP2 is a hallmark protein of the 20S U5 snRNP, absent from the mature tri-snRNP. Here we report a high-resolution cryogenic electron microscopy structure of the 20S U5 snRNP, shedding light on the mutually exclusive interfaces utilized during tri-snRNP assembly and the role of the CD2BP2 in facilitating this process.

PubMed: 38467877
DOI: 10.1038/s41594-024-01250-5

実験手法

ELECTRON MICROSCOPY (3.1 Å)