8Q87

Structure of the G. gallus 80S rotated ribosome in complex with eEF2 and SERBP1

これはPDB形式変換不可エントリーです。

8Q87 の概要

• エントリーDOI: 10.2210/pdb8q87/pdb
• EMDBエントリー: 18169
• 分子名称: 28S rRNA, Large ribosomal subunit protein uL30, Large ribosomal subunit protein eL8, ... (89 entities in total)
• 機能のキーワード: translation, complex, eef2, serbp1, ribosome
• 由来する生物種: Gallus gallus (chicken); 詳細
• タンパク質・核酸の鎖数: 85
• 化学式量合計: 3822248.17

構造登録者

Nurullina, L.,Jenner, L.,Yusupov, M. (登録日: 2023-08-18, 公開日: 2024-08-28, 最終更新日: 2025-03-12)

主引用文献

Nurullina, L.,Terrosu, S.,Myasnikov, A.G.,Jenner, L.B.,Yusupov, M.
Cryo-EM structure of the inactive ribosome complex accumulated in chick embryo cells in cold-stress conditions.
Febs Lett., 598:537-547, 2024

PubMed Abstract: Here, we present the high-resolution structure of the Gallus gallus 80S ribosome obtained from cold-treated chicken embryos. The translationally inactive ribosome complex contains elongation factor eEF2 with GDP, SERPINE1 mRNA binding protein 1 (SERBP1) and deacylated tRNA in the P/E position, showing common features with complexes already described in mammals. Modeling of most expansion segments of G. gallus 28S ribosomal RNA allowed us to identify specific features in their structural organization and to describe areas where a marked difference between mammalian and avian ribosomes could shed light on the evolution of the expansion segments. This study provides the first structure of an avian ribosome, establishing a model for future structural and functional studies on the translational machinery in Aves.

PubMed: 38395592
DOI: 10.1002/1873-3468.14831

実験手法

ELECTRON MICROSCOPY (2.4 Å)