8Q7W

Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 3)

8Q7W の概要

• エントリーDOI: 10.2210/pdb8q7w/pdb
• 関連するPDBエントリー: 8Q7Q 8Q7V 8Q7X
• EMDBエントリー: 18235 18238 18239
• 分子名称: U5 snRNA, Small nuclear ribonucleoprotein-associated proteins B and B', Small nuclear ribonucleoprotein Sm D2, ... (17 entities in total)
• 機能のキーワード: u5 snrnp, cd2bp2, tssc4, spliceosome, splicing
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 1040743.92

構造登録者

Riabov Bassat, D.,Plaschka, C.,Vorlaender, M.K. (登録日: 2023-08-17, 公開日: 2024-04-17, 最終更新日: 2024-05-29)

主引用文献

Riabov Bassat, D.,Visanpattanasin, S.,Vorlander, M.K.,Fin, L.,Phillips, A.W.,Plaschka, C.
Structural basis of human U5 snRNP late biogenesis and recycling.
Nat.Struct.Mol.Biol., 31:747-751, 2024

PubMed Abstract: Pre-mRNA splicing by the spliceosome requires the biogenesis and recycling of its small nuclear ribonucleoprotein (snRNP) complexes, which are consumed in each round of splicing. The human U5 snRNP is the ~1 MDa 'heart' of the spliceosome and is recycled through an unknown mechanism involving major architectural rearrangements and the dedicated chaperones CD2BP2 and TSSC4. Late steps in U5 snRNP biogenesis similarly involve these chaperones. Here we report cryo-electron microscopy structures of four human U5 snRNP-CD2BP2-TSSC4 complexes, revealing how a series of molecular events primes the U5 snRNP to generate the ~2 MDa U4/U6.U5 tri-snRNP, the largest building block of the spliceosome.

PubMed: 38467876
DOI: 10.1038/s41594-024-01243-4

実験手法

ELECTRON MICROSCOPY (3.9 Å)