8Q6M

Human SOD1 low dose data collecton

8Q6M の概要

• エントリーDOI: 10.2210/pdb8q6m/pdb
• 関連するPDBエントリー: 8CCX
• 分子名称: Superoxide dismutase [Cu-Zn], ACETATE ION, ZINC ION, ... (6 entities in total)
• 機能のキーワード: sod1, amyotrophic lateral sclerosis, als, pharmacological chaperone, cross-linking, stabilization, oxidoreductase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32522.66

構造登録者

Antonyuk, S.V.,Hossain, A.,Agar, J.N.,Hasnain, S.S. (登録日: 2023-08-14, 公開日: 2023-12-13, 最終更新日: 2024-02-07)

主引用文献

Hossain, M.A.,Sarin, R.,Donnelly, D.P.,Miller, B.C.,Weiss, A.,McAlary, L.,Antonyuk, S.V.,Salisbury, J.P.,Amin, J.,Conway, J.B.,Watson, S.S.,Winters, J.N.,Xu, Y.,Alam, N.,Brahme, R.R.,Shahbazian, H.,Sivasankar, D.,Padmakumar, S.,Sattarova, A.,Ponmudiyan, A.C.,Gawde, T.,Verrill, D.E.,Yang, W.,Kannapadi, S.,Plant, L.D.,Auclair, J.R.,Makowski, L.,Petsko, G.A.,Ringe, D.,Agar, N.Y.R.,Greenblatt, D.J.,Ondrechen, M.J.,Chen, Y.,Yerbury, J.J.,Manetsch, R.,Hasnain, S.S.,Brown Jr., R.H.,Agar, J.N.
Evaluating protein cross-linking as a therapeutic strategy to stabilize SOD1 variants in a mouse model of familial ALS.
Plos Biol., 22:e3002462-e3002462, 2024

PubMed Abstract: Mutations in the gene encoding Cu-Zn superoxide dismutase 1 (SOD1) cause a subset of familial amyotrophic lateral sclerosis (fALS) cases. A shared effect of these mutations is that SOD1, which is normally a stable dimer, dissociates into toxic monomers that seed toxic aggregates. Considerable research effort has been devoted to developing compounds that stabilize the dimer of fALS SOD1 variants, but unfortunately, this has not yet resulted in a treatment. We hypothesized that cyclic thiosulfinate cross-linkers, which selectively target a rare, 2 cysteine-containing motif, can stabilize fALS-causing SOD1 variants in vivo. We created a library of chemically diverse cyclic thiosulfinates and determined structure-cross-linking-activity relationships. A pre-lead compound, "S-XL6," was selected based upon its cross-linking rate and drug-like properties. Co-crystallographic structure clearly establishes the binding of S-XL6 at Cys 111 bridging the monomers and stabilizing the SOD1 dimer. Biophysical studies reveal that the degree of stabilization afforded by S-XL6 (up to 24°C) is unprecedented for fALS, and to our knowledge, for any protein target of any kinetic stabilizer. Gene silencing and protein degrading therapeutic approaches require careful dose titration to balance the benefit of diminished fALS SOD1 expression with the toxic loss-of-enzymatic function. We show that S-XL6 does not share this liability because it rescues the activity of fALS SOD1 variants. No pharmacological agent has been proven to bind to SOD1 in vivo. Here, using a fALS mouse model, we demonstrate oral bioavailability; rapid engagement of SOD1G93A by S-XL6 that increases SOD1G93A's in vivo half-life; and that S-XL6 crosses the blood-brain barrier. S-XL6 demonstrated a degree of selectivity by avoiding off-target binding to plasma proteins. Taken together, our results indicate that cyclic thiosulfinate-mediated SOD1 stabilization should receive further attention as a potential therapeutic approach for fALS.

PubMed: 38289969
DOI: 10.1371/journal.pbio.3002462

実験手法

X-RAY DIFFRACTION (1.77 Å)