8Q36

Structure of Nucleosome Core with a Bound Metallopeptide Conjugate (Foamy Virus GAG Peptide-Au[I] Compound)

これはPDB形式変換不可エントリーです。

8Q36 の概要

• エントリーDOI: 10.2210/pdb8q36/pdb
• 分子名称: GAG structural protein, Histone H3.1, Histone H4, ... (8 entities in total)
• 機能のキーワード: nucleosome, viral protein, metallopeptide conjugate, gold compound, dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 179296.24

構造登録者

De Falco, L.,Batchelor, L.K.,Dyson, P.J.,Davey, C.A. (登録日: 2023-08-03, 公開日: 2024-03-27)

主引用文献

Batchelor, L.K.,De Falco, L.,Dyson, P.J.,Davey, C.A.
Viral peptide conjugates for metal-warhead delivery to chromatin.
Rsc Adv, 14:8718-8725, 2024

PubMed Abstract: The presence of heavy metal groups can endow compounds with unique structural and chemical attributes beneficial for developing highly potent therapeutic agents and effective molecular labels. However, metallocompound binding site specificity is a major challenge that dictates the level of off-site targeting, which is a limiting factor in finding safer and more effective metal-based drugs. Here we designed and tested a family of metallopeptide conjugates based on two different chromatin-tethering viral proteins and a drug being repurposed for cancer, the Au(i) anti-arthritic auranofin. The viral peptides associate with the acidic patch of the nucleosome while the gold moiety can bind allosterically to the H3 H113 imidazole. To achieve synthesis of the conjugates, we also engineered a sulfur-free, nucleosome-binding Kaposi's sarcoma herpesvirus LANA peptide with a methionine-to-ornithine substitution and coupled the peptide to the metal group in a final step using click chemistry. The four conjugates tested are all selectively cytotoxic towards tumor cell lines, but the choice of viral peptide and mode of linkage to the Au(i) group influences metal binding site preference. Our findings suggest that viral peptide-metalloconjugates have potential for use in chromatin delivery of therapeutic warheads and as nucleosome-specific tags.

PubMed: 38495982
DOI: 10.1039/d4ra01617c

実験手法

X-RAY DIFFRACTION (2.604 Å)