8Q30

Sulfolobus acidocaldarius AAP filament.

これはPDB形式変換不可エントリーです。

8Q30 の概要

• エントリーDOI: 10.2210/pdb8q30/pdb
• EMDBエントリー: 18119
• 分子名称: DUF4352 domain-containing protein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 6-deoxy-6-sulfo-beta-D-glucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: cell surface appendage, n-glycosylation, protein fibril
• 由来する生物種: Sulfolobus acidocaldarius
• タンパク質・核酸の鎖数: 72
• 化学式量合計: 1108644.14

構造登録者

Isupov, M.N.,Gaines, M.,Daum, B.,McLaren, M. (登録日: 2023-08-03, 公開日: 2024-03-06, 最終更新日: 2024-10-23)

主引用文献

Gaines, M.C.,Sivabalasarma, S.,Isupov, M.N.,Haque, R.U.,McLaren, M.,Hanus, C.,Gold, V.A.M.,Albers, S.V.,Daum, B.
CryoEM reveals the structure of an archaeal pilus involved in twitching motility.
Nat Commun, 15:5050-5050, 2024

PubMed Abstract: Amongst the major types of archaeal filaments, several have been shown to closely resemble bacterial homologues of the Type IV pili (T4P). Within Sulfolobales, member species encode for three types of T4P, namely the archaellum, the UV-inducible pilus system (Ups) and the archaeal adhesive pilus (Aap). Whereas the archaellum functions primarily in swimming motility, and the Ups in UV-induced cell aggregation and DNA-exchange, the Aap plays an important role in adhesion and twitching motility. Here, we present a cryoEM structure of the Aap of the archaeal model organism Sulfolobus acidocaldarius. We identify the component subunit as AapB and find that while its structure follows the canonical T4P blueprint, it adopts three distinct conformations within the pilus. The tri-conformer Aap structure that we describe challenges our current understanding of pilus structure and sheds new light on the principles of twitching motility.

PubMed: 38877033
DOI: 10.1038/s41467-024-45831-w

実験手法

ELECTRON MICROSCOPY (3.22 Å)