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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Sulfolobus acidocaldarius AAP filament. | |||||||||
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![]() | cell surface appendage / ![]() | |||||||||
Function / homology | Immunoglobulin-like fold / ![]() ![]() | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | helical reconstruction / ![]() | |||||||||
![]() | Isupov MN / Gaines M / Daum B / McLaren M | |||||||||
Funding support | European Union, 1 items
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![]() | ![]() Title: CryoEM reveals the structure of an archaeal pilus involved in twitching motility Authors: Isupov MN / Gaines M / Daum B / McLaren M / Sivabalasarma S / Haque R / Hanus C / Gold V / Albers SV | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 24.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.1 KB 16.1 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 16.9 KB | Display | ![]() |
Images | ![]() | 82.2 KB | ||
Filedesc metadata | ![]() | 5.3 KB | ||
Others | ![]() ![]() ![]() | 443.5 MB 475.9 MB 475.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8q30MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 1.047 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Additional map: #1
File | emd_18119_additional_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_18119_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: DeepEMhance map
File | emd_18119_half_map_2.map | ||||||||||||
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Annotation | DeepEMhance map | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Filament
Entire | Name: Filament![]() |
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Components |
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-Supramolecule #1: Filament
Supramolecule | Name: Filament / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() ![]() |
-Macromolecule #1: DUF4352 domain-containing protein
Macromolecule | Name: DUF4352 domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 72 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 14.177022 KDa |
Sequence | String: LSGAIVALIL VIAGVIIAIA VVLFAFGLIP GISNQGSIQV LGSGTITNST ASGSSRTIYN ITITVKNTGT TSISVTSINI NGQPFNING TAPSIPAGRT QPITFEVTPA SGKPNFSPGA SYTATIYFSN GQGAPATLIY QG UniProtKB: DUF4352 domain-containing protein |
-Macromolecule #4: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 4 / Number of copies: 72 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Experimental details
-Structure determination
Method | ![]() |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 3 |
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Vitrification | Cryogen name: ETHANE |
Details | Mixed population of filaments isolated from Sulfolobus acidocaldarius |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number real images: 6272 / Average electron dose: 42.33 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Details | Jligand was used for preparing dictionaries for an unusual sugars |
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Refinement | Space: RECIPROCAL / Protocol: AB INITIO MODEL |
Output model | ![]() PDB-8q30: |