8Q1U

Inward-facing, open1 proteoliposome complex I at 3.3 A, after deactivation treatment. Initially purified in LMNG.

8Q1U の概要

• エントリーDOI: 10.2210/pdb8q1u/pdb
• 関連するPDBエントリー: 8Q1P
• EMDBエントリー: 18066 18067
• 分子名称: NADH-ubiquinone oxidoreductase chain 3, NADH-ubiquinone oxidoreductase chain 6, NADH-ubiquinone oxidoreductase chain 4L, ... (60 entities in total)
• 機能のキーワード: complex i, oxidoreductase, proteoliposomes, membrane-bound, metabolism, membrane protein
• 由来する生物種: Bos taurus (cattle); 詳細
• タンパク質・核酸の鎖数: 45
• 化学式量合計: 1096430.94

構造登録者

Grba, D.N.,Hirst, J. (登録日: 2023-08-01, 公開日: 2024-06-05, 最終更新日: 2024-06-26)

主引用文献

Grba, D.N.,Wright, J.J.,Yin, Z.,Fisher, W.,Hirst, J.
Molecular mechanism of the ischemia-induced regulatory switch in mammalian complex I.
Science, 384:1247-1253, 2024

PubMed Abstract: Respiratory complex I is an efficient driver for oxidative phosphorylation in mammalian mitochondria, but its uncontrolled catalysis under challenging conditions leads to oxidative stress and cellular damage. Ischemic conditions switch complex I from rapid, reversible catalysis into a dormant state that protects upon reoxygenation, but the molecular basis for the switch is unknown. We combined precise biochemical definition of complex I catalysis with high-resolution cryo-electron microscopy structures in the phospholipid bilayer of coupled vesicles to reveal the mechanism of the transition into the dormant state, modulated by membrane interactions. By implementing a versatile membrane system to unite structure and function, attributing catalytic and regulatory properties to specific structural states, we define how a conformational switch in complex I controls its physiological roles.

PubMed: 38870289
DOI: 10.1126/science.ado2075

実験手法

ELECTRON MICROSCOPY (3.3 Å)