8PX4

Structure of the PAS domain code by the LIC_11128 gene from Leptospira interrogans serovar Copenhageni Fiocruz, solved at wavelength 3.09 A

Summary for 8PX4

• Entry DOI: 10.2210/pdb8px4/pdb
• Descriptor: Diguanylate cyclase (2 entities in total)
• Functional Keywords: ggdef family protein, unknown function
• Biological source: Leptospira interrogans serovar Copenhageni
• Total number of polymer chains: 3
• Total formula weight: 40323.16

Authors

El Omari, K.,Duman, R.,Mykhaylyk, V.,Orr, C.,Guzzo, C.R.,Owens, R.J.,Wagner, A. (deposition date: 2023-07-22, release date: 2023-10-25)

Primary citation

El Omari, K.,Duman, R.,Mykhaylyk, V.,Orr, C.M.,Latimer-Smith, M.,Winter, G.,Grama, V.,Qu, F.,Bountra, K.,Kwong, H.S.,Romano, M.,Reis, R.I.,Vogeley, L.,Vecchia, L.,Owen, C.D.,Wittmann, S.,Renner, M.,Senda, M.,Matsugaki, N.,Kawano, Y.,Bowden, T.A.,Moraes, I.,Grimes, J.M.,Mancini, E.J.,Walsh, M.A.,Guzzo, C.R.,Owens, R.J.,Jones, E.Y.,Brown, D.G.,Stuart, D.I.,Beis, K.,Wagner, A.
Experimental phasing opportunities for macromolecular crystallography at very long wavelengths.
Commun Chem, 6:219-219, 2023

PubMed Abstract: Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength. Use of longer wavelengths to access the absorption edges of biologically important lighter atoms such as calcium, potassium, chlorine, sulfur and phosphorus for native-SAD phasing is attractive but technically highly challenging. The long-wavelength beamline I23 at Diamond Light Source overcomes these limitations and extends the accessible wavelength range to λ = 5.9 Å. Here we report 22 macromolecular structures solved in this extended wavelength range, using anomalous scattering from a range of elements which demonstrate the routine feasibility of lighter atom phasing. We suggest that, in light of its advantages, long-wavelength crystallography is a compelling option for experimental phasing.

PubMed: 37828292
DOI: 10.1038/s42004-023-01014-0

Experimental method

X-RAY DIFFRACTION (2.5 Å)