8PUN

Old Yellow Enzyme from the thermophilic Ferrovum sp. JA12

8PUN の概要

• エントリーDOI: 10.2210/pdb8pun/pdb
• 分子名称: NADPH dehydrogenase, FLAVIN MONONUCLEOTIDE, DI(HYDROXYETHYL)ETHER, ... (10 entities in total)
• 機能のキーワード: oye, thermophilic, ene-reductase, fmn, flavoenzyme, oxidoreductase
• 由来する生物種: Ferrovum sp. JA12
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 166829.30

構造登録者

Polidori, N.,Gruber, K. (登録日: 2023-07-17, 公開日: 2023-08-02, 最終更新日: 2025-05-14)

主引用文献

Polidori, N.,Babin, P.,Daniel, B.,Gruber, K.
Structure, Oligomerization, and Thermal Stability of a Recently Discovered Old Yellow Enzyme.
Proteins, 93:1181-1188, 2025

PubMed Abstract: The Old Yellow Enzyme from Ferrovum sp. JA12 (FOYE) displays an unusual thermal stability for an enzyme isolated from a mesophilic organism. We determined the crystal structure of this enzyme and performed bioinformatic characterization to get insights into its thermal stability. The enzyme displays a tetrameric quaternary structure; however, unlike the other tetrameric homologs, it clusters in a separate phylogenetic group and possesses unique interactions that stabilize this oligomeric state. The thermal stability of this enzyme is mainly due to an unusually high number of intramolecular hydrogen bonds. Finally, this study provides a general analysis of the forces driving the oligomerization in Old Yellow Enzymes.

PubMed: 39840754
DOI: 10.1002/prot.26800

実験手法

X-RAY DIFFRACTION (2.3 Å)