8PRK

THE R78K AND D117E ACTIVE SITE VARIANTS OF SACCHAROMYCES CEREVISIAE SOLUBLE INORGANIC PYROPHOSPHATASE: STRUCTURAL STUDIES AND MECHANISTIC IMPLICATIONS

Summary for 8PRK

Entry DOI	10.2210/pdb8prk/pdb
Descriptor	PROTEIN (INORGANIC PYROPHOSPHATASE), MANGANESE (II) ION, PHOSPHATE ION, ... (4 entities in total)
Functional Keywords	enzyme mechanism, iorganic pyrophosphatase, mutant structures, 2-metal ion mechanism, hydrolase
Biological source	Saccharomyces cerevisiae (baker's yeast)
Cellular location	Cytoplasm: P00817
Total number of polymer chains	2
Total formula weight	65176.68
Authors	Tuominen, V.,Heikinheimo, P.,Kajander, T.,Torkkel, T.,Hyytia, T.,Kapyla, J.,Lahti, R.,Cooperman, B.S.,Goldman, A. (deposition date: 1998-09-16, release date: 1998-12-23, Last modification date: 2023-09-20)
Primary citation	Tuominen, V.,Heikinheimo, P.,Kajander, T.,Torkkel, T.,Hyytia, T.,Kapyla, J.,Lahti, R.,Cooperman, B.S.,Goldman, A. The R78K and D117E active-site variants of Saccharomyces cerevisiae soluble inorganic pyrophosphatase: structural studies and mechanistic implications. J.Mol.Biol., 284:1565-1580, 1998 Cited by PubMed: 9878371 DOI: 10.1006/jmbi.1998.2266 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.85 Å)

Structure validation