8PK0

human mitoribosomal large subunit assembly intermediate 1 with GTPBP10-GTPBP7

This is a non-PDB format compatible entry.

Summary for 8PK0

• Entry DOI: 10.2210/pdb8pk0/pdb
• EMDB information: 17719
• Descriptor: 39S ribosomal protein L32, mitochondrial, 39S ribosomal protein L35, mitochondrial, 39S ribosomal protein L27, mitochondrial, ... (64 entities in total)
• Functional Keywords: mitochondria, maturation, biogenesis, ribosome, gtpbp7, gtpbp10, gtp binding protein 10, obgh2, mtg1, lsu
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 58
• Total formula weight: 1963042.98

Authors

Kummer, E.,Nguyen, T.G.,Ritter, C. (deposition date: 2023-06-23, release date: 2023-12-13, Last modification date: 2024-06-26)

Primary citation

Nguyen, T.G.,Ritter, C.,Kummer, E.
Structural insights into the role of GTPBP10 in the RNA maturation of the mitoribosome.
Nat Commun, 14:7991-7991, 2023

PubMed Abstract: Mitochondria contain their own genetic information and a dedicated translation system to express it. The mitochondrial ribosome is assembled from mitochondrial-encoded RNA and nuclear-encoded ribosomal proteins. Assembly is coordinated in the mitochondrial matrix by biogenesis factors that transiently associate with the maturing particle. Here, we present a structural snapshot of a large mitoribosomal subunit assembly intermediate containing 7 biogenesis factors including the GTPases GTPBP7 and GTPBP10. Our structure illustrates how GTPBP10 aids the folding of the ribosomal RNA during the biogenesis process, how this process is related to bacterial ribosome biogenesis, and why mitochondria require two biogenesis factors in contrast to only one in bacteria.

PubMed: 38042949
DOI: 10.1038/s41467-023-43599-z

Experimental method

ELECTRON MICROSCOPY (3.03 Å)