8PJC
Crystal structure of human insulin desB30 precursor with an Alanine-Alanine-Lysine C-peptide in dimer (T2) conformation
Summary for 8PJC
| Entry DOI | 10.2210/pdb8pjc/pdb |
| Descriptor | Insulin (2 entities in total) |
| Functional Keywords | insulin, precursor, dimer, hormone |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 5969.87 |
| Authors | Schluckebier, G.,Johansson, E. (deposition date: 2023-06-23, release date: 2023-11-08, Last modification date: 2024-10-16) |
| Primary citation | Kjeldsen, T.,Andersen, A.S.,Hubalek, F.,Johansson, E.,Kreiner, F.F.,Schluckebier, G.,Kurtzhals, P. Molecular engineering of insulin for recombinant expression in yeast. Trends Biotechnol, 42:464-478, 2024 Cited by PubMed Abstract: Since the first administration of insulin to a person with diabetes in 1922, scientific contributions from academia and industry have improved insulin therapy and access. The pharmaceutical need for insulin is now more than 40 tons annually, half of which is produced by recombinant secretory expression in Saccharomyces cerevisiae. We discuss how, in this yeast species, adaptation of insulin precursors by removable structural elements is pivotal for efficient secretory expression. The technologies reviewed have been implemented at industrial scale and are seminal for the supply of human insulin and insulin analogues to people with diabetes now and in the future. Engineering of a target protein with removable structural elements may provide a general approach to yield optimisation. PubMed: 37880066DOI: 10.1016/j.tibtech.2023.09.012 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.14 Å) |
Structure validation
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