8PJ9

Cas9 bound to cognate DNA, Streptococcus thermophilus DGCC 7710 CRISPR3 system

8PJ9 の概要

• エントリーDOI: 10.2210/pdb8pj9/pdb
• 関連するPDBエントリー: 8PIG
• EMDBエントリー: 17702
• 分子名称: CRISPR-associated endonuclease Cas9, crRNA, chain B, tracrRNA, chain C, ... (6 entities in total)
• 機能のキーワード: cas9, crispr-cas, pam, spacer acquisition, rna binding protein
• 由来する生物種: Streptococcus thermophilus DGCC 7710; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 223310.10

構造登録者

Sasnauskas, G.,Gaizauskaite, U.,Tamulaitiene, G. (登録日: 2023-06-23, 公開日: 2024-07-10, 最終更新日: 2026-03-04)

主引用文献

Gaizauskaite, U.,Tamulaitiene, G.,Silanskas, A.,Gasiunas, G.,Siksnys, V.,Sasnauskas, G.
Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation.
Mol.Cell, 2026

PubMed Abstract: During CRISPR-Cas adaptation, prokaryotic cells become immunized by the insertion of foreign DNA fragments, termed spacers, into the host genome to serve as templates for RNA-guided immunity. Spacer acquisition relies on the Cas1-Cas2 integrase and accessory proteins, which select DNA sequences flanked by the protospacer adjacent motif (PAM) and insert them into the CRISPR array. It has been shown that in type II-A systems, selection of PAM-proximal prespacers is mediated by the effector nuclease Cas9, which forms a "supercomplex" with the Cas1-Cas2 integrase and the Csn2 protein. Here, we present cryo-electron microscopy structures of the Streptococcus thermophilus type II-A prespacer selection supercomplex in the DNA-scanning and two distinct PAM-bound configurations, providing insights into the mechanism of Cas9-mediated prespacer selection in type II-A CRISPR-Cas systems. Repurposing Cas9 by the CRISPR adaptation machinery for prespacer selection, as characterized here, demonstrates Cas9 plasticity and expands our knowledge of Cas9 biology.

PubMed: 41702403
DOI: 10.1016/j.molcel.2026.01.022

実験手法

ELECTRON MICROSCOPY (3.24 Å)