8PFK

RNA structure with 1-methylpseudouridine, C2 space group

8PFK の概要

• エントリーDOI: 10.2210/pdb8pfk/pdb
• 分子名称: RNA (12-mer), MAGNESIUM ION (3 entities in total)
• 機能のキーワード: sars-cov-2, covid-19, coronavirus, mrna vaccine, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3914.00

構造登録者

Spingler, B.,McAuley, K.,Nievergelt, P.,Thorn, A. (登録日: 2023-06-16, 公開日: 2024-01-31, 最終更新日: 2025-02-19)

主引用文献

Nievergelt, P.,Berliat, F.,McAuley, K.E.,Dorgan, C.R.,van Well, R.M.,Thorn, A.,Spingler, B.
RNA oligomers at atomic resolution containing 1-methylpseudouridine, an essential building block of mRNA vaccines.
Chemmedchem, 19:e202300600-e202300600, 2024

PubMed Abstract: All widely used mRNA vaccines against COVID-19 contain in their sequence 1-methylpseudouridine (m1Ψ) instead of uridine. In this publication, we report two high resolution crystal structures (at up to 1.01 and 1.32 Å, respectively) of one such double-stranded 12-mer RNA sequence crystallized in two crystal forms. The structures are compared with similar structures which do not contain this modification. Additionally, the X-ray structure of 1-methyl-pseudouridine itself was determined.

PubMed: 38235959
DOI: 10.1002/cmdc.202300600

実験手法

X-RAY DIFFRACTION (1.321 Å)