8PFH

Crystal structure of the yeast septin complex Shs1-Cdc12-Cdc3-Cdc10

8PFH の概要

• エントリーDOI: 10.2210/pdb8pfh/pdb
• 分子名称: CDC10 isoform 1, Cell division control protein 3, CDC12 isoform 1, ... (5 entities in total)
• 機能のキーワード: septins, complex, structural protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 148970.46

構造登録者

Grupp, B.,Denkhaus, L.,Gerhardt, S.,Gronemeyer, T. (登録日: 2023-06-16, 公開日: 2023-12-06, 最終更新日: 2024-02-21)

主引用文献

Grupp, B.,Denkhaus, L.,Gerhardt, S.,Vogele, M.,Johnsson, N.,Gronemeyer, T.
The structure of a tetrameric septin complex reveals a hydrophobic element essential for NC-interface integrity.
Commun Biol, 7:48-48, 2024

PubMed Abstract: The septins of the yeast Saccharomyces cerevisiae assemble into hetero-octameric rods by alternating interactions between neighboring G-domains or N- and C-termini, respectively. These rods polymerize end to end into apolar filaments, forming a ring beneath the prospective new bud that expands during the cell cycle into an hourglass structure. The hourglass finally splits during cytokinesis into a double ring. Understanding these transitions as well as the plasticity of the higher order assemblies requires a detailed knowledge of the underlying structures. Here we present the first X-ray crystal structure of a tetrameric Shs1-Cdc12-Cdc3-Cdc10 complex at a resolution of 3.2 Å. Close inspection of the NC-interfaces of this and other septin structures reveals a conserved contact motif that is essential for NC-interface integrity of yeast and human septins in vivo and in vitro. Using the tetrameric structure in combination with AlphaFold-Multimer allowed us to propose a model of the octameric septin rod.

PubMed: 38184752
DOI: 10.1038/s42003-023-05734-w

実験手法

X-RAY DIFFRACTION (3.24 Å)