8PEI
Crystal structure of the biphotochromic fluorescent protein SAASoti (C21N/V127T variant) in its green on-state
Summary for 8PEI
| Entry DOI | 10.2210/pdb8pei/pdb |
| Descriptor | C21N/V127T form of the biphotochromic fluorescent protein SAASoti (2 entities in total) |
| Functional Keywords | saasoti, biphotoswitchable form, chromophore, green state, photoswitching, nonfluorescent state, photoconversion, fluorescent protein, photoconvertible fluorescent protein, reversible photoswitching |
| Biological source | Stylocoeniella armata |
| Total number of polymer chains | 2 |
| Total formula weight | 52519.50 |
| Authors | Boyko, K.M.,Varfolomeeva, L.A.,Matyuta, I.O.,Gavshina, A.V.,Solovyev, I.D.,Popov, V.O.,Savitsky, A.P. (deposition date: 2023-06-14, release date: 2024-01-17, Last modification date: 2024-10-16) |
| Primary citation | Gavshina, A.V.,Solovyev, I.D.,Khrenova, M.G.,Boyko, K.M.,Varfolomeeva, L.A.,Minyaev, M.E.,Popov, V.O.,Savitsky, A.P. The role of the correlated motion(s) of the chromophore in photoswitching of green and red forms of the photoconvertible fluorescent protein mSAASoti. Sci Rep, 14:8754-8754, 2024 Cited by PubMed Abstract: Wild-type SAASoti and its monomeric variant mSAASoti can undergo phototransformations, including reversible photoswitching of the green form to a nonfluorescent state and irreversible green-to-red photoconversion. In this study, we extend the photochemistry of mSAASoti variants to enable reversible photoswitching of the red form. This result is achieved by rational and site-saturated mutagenesis of the M163 and F177 residues. In the case of mSAASoti it is M163T substitution that leads to the fastest switching and the most photostable variant, and reversible photoswitching can be observed for both green and red forms when expressed in eukaryotic cells. We obtained a 13-fold increase in the switching efficiency with the maximum switching contrast of the green form and the appearance of comparable switching of the red form for the C21N/M163T mSAASoti variant. The crystal structure of the C21N mSAASoti in its green on-state was obtained for the first time at 3.0 Å resolution, and it is in good agreement with previously calculated 3D-model. Dynamic network analysis reveals that efficient photoswitching occurs if motions of the 66H residue and phenyl fragment of chromophore are correlated and these moieties belong to the same community. PubMed: 38627478DOI: 10.1038/s41598-024-59364-1 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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