8PEI

Crystal structure of the biphotochromic fluorescent protein SAASoti (C21N/V127T variant) in its green on-state

8PEI の概要

• エントリーDOI: 10.2210/pdb8pei/pdb
• 分子名称: C21N/V127T form of the biphotochromic fluorescent protein SAASoti (2 entities in total)
• 機能のキーワード: saasoti, biphotoswitchable form, chromophore, green state, photoswitching, nonfluorescent state, photoconversion, fluorescent protein, photoconvertible fluorescent protein, reversible photoswitching
• 由来する生物種: Stylocoeniella armata
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52519.50

構造登録者

Boyko, K.M.,Varfolomeeva, L.A.,Matyuta, I.O.,Gavshina, A.V.,Solovyev, I.D.,Popov, V.O.,Savitsky, A.P. (登録日: 2023-06-14, 公開日: 2024-01-17, 最終更新日: 2024-10-16)

主引用文献

Gavshina, A.V.,Solovyev, I.D.,Khrenova, M.G.,Boyko, K.M.,Varfolomeeva, L.A.,Minyaev, M.E.,Popov, V.O.,Savitsky, A.P.
The role of the correlated motion(s) of the chromophore in photoswitching of green and red forms of the photoconvertible fluorescent protein mSAASoti.
Sci Rep, 14:8754-8754, 2024

PubMed Abstract: Wild-type SAASoti and its monomeric variant mSAASoti can undergo phototransformations, including reversible photoswitching of the green form to a nonfluorescent state and irreversible green-to-red photoconversion. In this study, we extend the photochemistry of mSAASoti variants to enable reversible photoswitching of the red form. This result is achieved by rational and site-saturated mutagenesis of the M163 and F177 residues. In the case of mSAASoti it is M163T substitution that leads to the fastest switching and the most photostable variant, and reversible photoswitching can be observed for both green and red forms when expressed in eukaryotic cells. We obtained a 13-fold increase in the switching efficiency with the maximum switching contrast of the green form and the appearance of comparable switching of the red form for the C21N/M163T mSAASoti variant. The crystal structure of the C21N mSAASoti in its green on-state was obtained for the first time at 3.0 Å resolution, and it is in good agreement with previously calculated 3D-model. Dynamic network analysis reveals that efficient photoswitching occurs if motions of the 66H residue and phenyl fragment of chromophore are correlated and these moieties belong to the same community.

PubMed: 38627478
DOI: 10.1038/s41598-024-59364-1

実験手法

X-RAY DIFFRACTION (3 Å)