8P6G

Crystal structure of the improved version of the Genetically Encoded Green Calcium Indicator YTnC2-5

Summary for 8P6G

• Entry DOI: 10.2210/pdb8p6g/pdb
• Descriptor: Genetically Encoded Green Calcium Indicator YTnC2-5 based on Troponin C from toadfish, DI(HYDROXYETHYL)ETHER (3 entities in total)
• Functional Keywords: calcium sensor, indicator, tnc, troponin, geci, ytnc2, fluorescent protein
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 71858.08

Authors

Boyko, K.M.,Nikolaeva, A.Y.,Subach, O.M.,Vlaskina, A.V.,Agapova, Y.K.,Popov, V.O.,Subach, F.V. (deposition date: 2023-05-26, release date: 2024-04-03, Last modification date: 2024-11-13)

Primary citation

Subach, O.M.,Vlaskina, A.V.,Agapova, Y.K.,Nikolaeva, A.Y.,Varizhuk, A.M.,Podgorny, O.V.,Piatkevich, K.D.,Patrushev, M.V.,Boyko, K.M.,Subach, F.V.
YTnC2, an improved genetically encoded green calcium indicator based on toadfish troponin C.
Febs Open Bio, 13:2047-2060, 2023

PubMed Abstract: Genetically encoded calcium indicators based on truncated troponin C are attractive probes for calcium imaging due to their relatively small molecular size and twofold reduced calcium ion buffering. However, the best-suited members of this family, YTnC and cNTnC, suffer from low molecular brightness, limited dynamic range, and/or poor sensitivity to calcium transients in neurons. To overcome these limitations, we developed an enhanced version of YTnC, named YTnC2. Compared with YTnC, YTnC2 had 5.7-fold higher molecular brightness and 6.4-fold increased dynamic range in vitro. YTnC2 was successfully used to reveal calcium transients in the cytosol and in the lumen of mitochondria of both mammalian cells and cultured neurons. Finally, we obtained and analyzed the crystal structure of the fluorescent domain of the YTnC2 mutant.

PubMed: 37650870
DOI: 10.1002/2211-5463.13702

Experimental method

X-RAY DIFFRACTION (1.95 Å)