8P60

Spraguea lophii ribosome dimer

これはPDB形式変換不可エントリーです。

「7QJH」から置き換えられました

8P60 の概要

• エントリーDOI: 10.2210/pdb8p60/pdb
• 関連するPDBエントリー: 7QCA
• EMDBエントリー: 17457
• 分子名称: RNA 28S, 60S ribosomal protein L6, 60S ribosomal protein L32, ... (76 entities in total)
• 機能のキーワード: microsporidia, ribosome
• 由来する生物種: Spraguea lophii 42_110; 詳細
• タンパク質・核酸の鎖数: 150
• 化学式量合計: 5294197.41

構造登録者

Gil Diez, P.,McLaren, M.,Isupov, M.N.,Daum, B.,Conners, R.,Williams, B. (登録日: 2023-05-24, 公開日: 2023-06-21, 最終更新日: 2024-11-13)

主引用文献

McLaren, M.,Conners, R.,Isupov, M.N.,Gil-Diez, P.,Gambelli, L.,Gold, V.A.M.,Walter, A.,Connell, S.R.,Williams, B.,Daum, B.
CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state.
Nat Microbiol, 8:1834-1845, 2023

PubMed Abstract: Translational control is an essential process for the cell to adapt to varying physiological or environmental conditions. To survive adverse conditions such as low nutrient levels, translation can be shut down almost entirely by inhibiting ribosomal function. Here we investigated eukaryotic hibernating ribosomes from the microsporidian parasite Spraguea lophii in situ by a combination of electron cryo-tomography and single-particle electron cryo-microscopy. We show that microsporidian spores contain hibernating ribosomes that are locked in a dimeric (100S) state, which is formed by a unique dimerization mechanism involving the beak region. The ribosomes within the dimer are fully assembled, suggesting that they are ready to be activated once the host cell is invaded. This study provides structural evidence for dimerization acting as a mechanism for ribosomal hibernation in microsporidia, and therefore demonstrates that eukaryotes utilize this mechanism in translational control.

PubMed: 37709902
DOI: 10.1038/s41564-023-01469-w

実験手法

ELECTRON MICROSCOPY (14.3 Å)