8P4K

Vaccinia Virus palisade layer A10 trimer

8P4K の概要

• エントリーDOI: 10.2210/pdb8p4k/pdb
• 関連するPDBエントリー: 8P4K
• EMDBエントリー: 17410 17411 17412 17413 17414
• 分子名称: Core protein OPG136 (1 entity in total)
• 機能のキーワード: poxvirus, vaccinia virus, core, cryo-electron tomography, alphafold, viral protein
• 由来する生物種: Vaccinia virus Western Reserve
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 207204.73

構造登録者

Datler, J.,Hansen, J.M.,Thader, A.,Schloegl, A.,Hodirnau, V.V.,Schur, F.K.M. (登録日: 2023-05-22, 公開日: 2024-01-17, 最終更新日: 2024-10-16)

主引用文献

Datler, J.,Hansen, J.M.,Thader, A.,Schlogl, A.,Bauer, L.W.,Hodirnau, V.V.,Schur, F.K.M.
Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores.
Nat.Struct.Mol.Biol., 31:1114-1123, 2024

PubMed Abstract: Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remained sparse. While major core proteins have been annotated via indirect experimental evidence, their structures have remained elusive and they could not be assigned to individual core features. Hence, which proteins constitute which layers of the core, such as the palisade layer and the inner core wall, has remained enigmatic. Here we show, using a multi-modal cryo-electron microscopy (cryo-EM) approach in combination with AlphaFold molecular modeling, that trimers formed by the cleavage product of VACV protein A10 are the key component of the palisade layer. This allows us to place previously obtained descriptions of protein interactions within the core wall into perspective and to provide a detailed model of poxvirus core architecture. Importantly, we show that interactions within A10 trimers are likely generalizable over members of orthopox- and parapoxviruses.

PubMed: 38316877
DOI: 10.1038/s41594-023-01201-6

実験手法

ELECTRON MICROSCOPY (3.8 Å)