8P2K

Ternary complex of translating ribosome, NAC and METAP1

これはPDB形式変換不可エントリーです。

8P2K の概要

• エントリーDOI: 10.2210/pdb8p2k/pdb
• EMDBエントリー: 17367
• 分子名称: 28S rRNA, 60S ribosomal protein L7a, 60S ribosomal protein L9, ... (97 entities in total)
• 機能のキーワード: ribosome, methionine aminopeptidase 1, methionine excision, protein biogenesis, nascent chain, translation
• 由来する生物種: Oryctolagus cuniculus (rabbit); 詳細
• タンパク質・核酸の鎖数: 88
• 化学式量合計: 3984430.61

構造登録者

Jia, M.,Jaskolowski, M.,Scaiola, A.,Jomaa, A.,Ban, N. (登録日: 2023-05-16, 公開日: 2023-07-19, 最終更新日: 2024-04-24)

主引用文献

Gamerdinger, M.,Jia, M.,Schloemer, R.,Rabl, L.,Jaskolowski, M.,Khakzar, K.M.,Ulusoy, Z.,Wallisch, A.,Jomaa, A.,Hunaeus, G.,Scaiola, A.,Diederichs, K.,Ban, N.,Deuerling, E.
NAC controls cotranslational N-terminal methionine excision in eukaryotes.
Science, 380:1238-1243, 2023

PubMed Abstract: N-terminal methionine excision from newly synthesized proteins, catalyzed cotranslationally by methionine aminopeptidases (METAPs), is an essential and universally conserved process that plays a key role in cell homeostasis and protein biogenesis. However, how METAPs interact with ribosomes and how their cleavage specificity is ensured is unknown. We discovered that in eukaryotes the nascent polypeptide-associated complex (NAC) controls ribosome binding of METAP1. NAC recruits METAP1 using a long, flexible tail and provides a platform for the formation of an active methionine excision complex at the ribosomal tunnel exit. This mode of interaction ensures the efficient excision of methionine from cytosolic proteins, whereas proteins targeted to the endoplasmic reticulum are spared. Our results suggest a broader mechanism for how access of protein biogenesis factors to translating ribosomes is controlled.

PubMed: 37347872
DOI: 10.1126/science.adg3297

実験手法

ELECTRON MICROSCOPY (2.9 Å)