8P1N

Structure of hantaan orthohantavirus (HTNV) polymerase bound to 5'vRNA

8P1N の概要

• エントリーDOI: 10.2210/pdb8p1n/pdb
• EMDBエントリー: 17355
• 分子名称: RNA-directed RNA polymerase L, RNA (5'-R(P*AP*GP*UP*AP*G)-3') (2 entities in total)
• 機能のキーワード: rna-dependent rna polymerase, htnv, hantaan orthohantavirus, bunyavirales, hantaviridae., viral protein
• 由来する生物種: Hantaan orthohantavirus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 257210.26

構造登録者

Keown, J.R.,Carrique, L.,Grimes, J.M. (登録日: 2023-05-12, 公開日: 2024-05-22, 最終更新日: 2025-01-08)

主引用文献

Keown, J.R.,Carrique, L.,Nilsson-Payant, B.E.,Fodor, E.,Grimes, J.M.
Structural characterization of the full-length Hantaan virus polymerase.
Plos Pathog., 20:e1012781-e1012781, 2024

PubMed Abstract: Hantaviridae are a family of segmented negative-sense RNA viruses that contain important human and animal pathogens. Hantaviridae contain a viral RNA-dependent RNA polymerase that replicates and transcribes the viral genome. Here we establish the expression and purification of the polymerase from the Old World Hantaan virus and characterise the structure using Cryo-EM. We determine a series of structures at resolutions between 2.7 and 3.3 Å of RNA free polymerase comprising the core, core and endonuclease, and a full-length polymerase. The full-length polymerase structure depicts the location of the cap binding and C-terminal domains which are arranged in a conformation that is incompatible with transcription and in a novel conformation not observed in previous conformations of cap-snatching viral polymerases. We further describe structures with 5' vRNA promoter in the presence and absence of a nucleotide triphosphate. The nucleotide bound structure mimics a replication pre-initiation complex and the nucleotide stabilises the motif E in a conformation distinct from those previously observed. We observe motif E in four distinct conformations including β-sheet, two helical arrangements, and nucleotide primed arrangement. The insights gained here guide future mechanistic studies of both the transcription and replication activities of the hantavirus polymerase and for the development of therapeutic targets.

PubMed: 39652621
DOI: 10.1371/journal.ppat.1012781

実験手法

ELECTRON MICROSCOPY (2.79 Å)