8OSI

Genetically encoded green ratiometric calcium indicator FNCaMP in calcium-bound state

8OSI の概要

• エントリーDOI: 10.2210/pdb8osi/pdb
• 分子名称: mNeonGreen,Calmodulin,Contig An16c0100, genomic contig, CALCIUM ION (3 entities in total)
• 機能のキーワード: fluorescent indicator, fluorescent sensor, chromophore, mneongreen, fluorescent protein
• 由来する生物種: Branchiostoma lanceolatum; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52420.45

構造登録者

Varfolomeeva, L.A.,Boyko, K.M.,Nikolaeva, A.Y.,Subach, O.M.,Subach, F.V. (登録日: 2023-04-19, 公開日: 2023-05-24, 最終更新日: 2024-10-23)

主引用文献

Subach, O.M.,Varfolomeeva, L.,Vlaskina, A.V.,Agapova, Y.K.,Nikolaeva, A.Y.,Piatkevich, K.D.,Patrushev, M.V.,Boyko, K.M.,Subach, F.V.
FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein.
Biochem.Biophys.Res.Commun., 665:169-177, 2023

PubMed Abstract: Neurobiologists widely use green genetically encoded calcium indicators (GECIs) for visualization of neuronal activity. Among them, ratiometric GECIs allow imaging of both active and non-active neuronal populations. However, they are not popular, since their properties are inferior to intensiometric GCaMP series of GECIs. The most characterized and developed ratiometric green GECI is FGCaMP7. However, the dynamic range and sensitivity of its large Stock's shift green (LSS-Green) form is significantly lower than its Green form and its molecular design is not optimal. To address these drawbacks, we engineered a ratiometric green calcium indicator, called FNCaMP, which is based on bright mNeonGreen protein and calmodulin from A. niger and has optimal NTnC-like design. We compared the properties of the FNCaMP and FGCaMP7 indicators in vitro, in mammalian cells, and in neuronal cultures. Finally, we obtained and analyzed X-ray structure of the FNCaMP indicator.

PubMed: 37163937
DOI: 10.1016/j.bbrc.2023.04.108

実験手法

X-RAY DIFFRACTION (2.42 Å)