8ORG

Cryo-EM structure of SH-SY5Y seeded with filaments from corticobasal degeneration extracts (Type II)

8ORG の概要

• エントリーDOI: 10.2210/pdb8org/pdb
• EMDBエントリー: 17123
• 分子名称: Microtubule-associated protein tau (1 entity in total)
• 機能のキーワード: amyloid, tau, protein fibril
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 129104.36

構造登録者

Lovestam, S.,Scheres, S.H.W.,Goedert, M. (登録日: 2023-04-13, 公開日: 2023-04-26, 最終更新日: 2024-07-24)

主引用文献

Tarutani, A.,Lovestam, S.,Zhang, X.,Kotecha, A.,Robinson, A.C.,Mann, D.M.A.,Saito, Y.,Murayama, S.,Tomita, T.,Goedert, M.,Scheres, S.H.W.,Hasegawa, M.
Cryo-EM structures of tau filaments from SH-SY5Y cells seeded with brain extracts from cases of Alzheimer's disease and corticobasal degeneration.
Febs Open Bio, 13:1394-1404, 2023

PubMed Abstract: The formation of amyloid filaments through templated seeding is believed to underlie the propagation of pathology in most human neurodegenerative diseases. A widely used model system to study this process is to seed amyloid filament formation in cultured cells using human brain extracts. Here, we report the electron cryo-microscopy structures of tau filaments from  undifferentiated seeded SH-SY5Y cells that transiently expressed N-terminally HA-tagged 1N3R or 1N4R human tau, using brain extracts from individuals with Alzheimer's disease or corticobasal degeneration. Although the resulting filament structures differed from those of the brain seeds, some degrees of structural templating were observed. Studying templated seeding in cultured cells, and determining the structures of the resulting filaments, can thus provide insights into the cellular aspects underlying neurodegenerative diseases.

PubMed: 37337995
DOI: 10.1002/2211-5463.13657

実験手法

ELECTRON MICROSCOPY (2.3 Å)