8OQ0

Crystal structure of tailspike depolymerase (APK09_gp48) from Acinetobacter phage APK09

8OQ0 の概要

• エントリーDOI: 10.2210/pdb8oq0/pdb
• 分子名称: Tailspike protein, DI(HYDROXYETHYL)ETHER (3 entities in total)
• 機能のキーワード: bacteriophage, acinetobacter baumannii, tailspike depolymerase, viral protein
• 由来する生物種: Acinetobacter phage APK09
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 66704.44

構造登録者

Matyuta, I.O.,Boyko, K.M.,Nikolaeva, A.Y.,Shneider, M.M.,Timoshina, O.Y.,Popova, A.V.,Miroshnikov, K.A.,Popov, V.O. (登録日: 2023-04-10, 公開日: 2023-05-31, 最終更新日: 2024-06-19)

主引用文献

Timoshina, O.Y.,Kasimova, A.A.,Shneider, M.M.,Matyuta, I.O.,Nikolaeva, A.Y.,Evseev, P.V.,Arbatsky, N.P.,Shashkov, A.S.,Chizhov, A.O.,Shelenkov, A.A.,Mikhaylova, Y.V.,Slukin, P.V.,Volozhantsev, N.V.,Boyko, K.M.,Knirel, Y.A.,Miroshnikov, K.A.,Popova, A.V.
Friunavirus Phage-Encoded Depolymerases Specific to Different Capsular Types of Acinetobacter baumannii .
Int J Mol Sci, 24:-, 2023

PubMed Abstract: is a critical priority nosocomial pathogen that produces a variety of capsular polysaccharides (CPSs), the primary receptors for specific depolymerase-carrying phages. In this study, the tailspike depolymerases (TSDs) encoded in genomes of six novel Friunaviruses, APK09, APK14, APK16, APK86, APK127v, APK128, and one previously described phage, APK37.1, were characterized. For all TSDs, the mechanism of specific cleavage of corresponding capsular polysaccharides (CPSs) was established. The structures of oligosaccharide fragments derived from K9, K14, K16, K37/K3-v1, K86, K127, and K128 CPSs degradation by the recombinant depolymerases have been determined. The crystal structures of three of the studied TSDs were obtained. A significant reduction in mortality of larvae infected with of K9 capsular type was shown in the example of recombinant TSD APK09_gp48. The data obtained will provide a better understanding of the interaction of phage-bacterial host systems and will contribute to the formation of principles of rational usage of lytic phages and phage-derived enzymes as antibacterial agents.

PubMed: 37240444
DOI: 10.3390/ijms24109100

実験手法

X-RAY DIFFRACTION (2.59 Å)