8OIT

39S human mitochondrial large ribosomal subunit with mtRF1 and P-site tRNA

これはPDB形式変換不可エントリーです。

8OIT の概要

• エントリーDOI: 10.2210/pdb8oit/pdb
• EMDBエントリー: 16894 16895 16896 16897 16898 16899
• 分子名称: 39S ribosomal protein L12, mitochondrial, 39S ribosomal protein L47, mitochondrial, 39S ribosomal protein L30, mitochondrial, ... (63 entities in total)
• 機能のキーワード: human mitochondrial ribosome, release factor mtrf1, non-canonical stop codon, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 62
• 化学式量合計: 1994472.90

構造登録者

Saurer, M.,Leibundgut, M.,Scaiola, A.,Schoenhut, T.,Ban, N. (登録日: 2023-03-23, 公開日: 2023-06-14, 最終更新日: 2024-06-26)

主引用文献

Saurer, M.,Leibundgut, M.,Nadimpalli, H.P.,Scaiola, A.,Schonhut, T.,Lee, R.G.,Siira, S.J.,Rackham, O.,Dreos, R.,Lenarcic, T.,Kummer, E.,Gatfield, D.,Filipovska, A.,Ban, N.
Molecular basis of translation termination at noncanonical stop codons in human mitochondria.
Science, 380:531-536, 2023

PubMed Abstract: The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the standard genetic code, including the reassignment of two arginine codons to stop codons. The protein required for translation termination at these noncanonical stop codons to release the newly synthesized polypeptides is not currently known. In this study, we used gene editing and ribosomal profiling in combination with cryo-electron microscopy to establish that mitochondrial release factor 1 (mtRF1) detects noncanonical stop codons in human mitochondria by a previously unknown mechanism of codon recognition. We discovered that binding of mtRF1 to the decoding center of the ribosome stabilizes a highly unusual conformation in the messenger RNA in which the ribosomal RNA participates in specific recognition of the noncanonical stop codons.

PubMed: 37141370
DOI: 10.1126/science.adf9890

実験手法

ELECTRON MICROSCOPY (2.9 Å)