8OI7

Trichomonas vaginalis riboside hydrolase

8OI7 の概要

• エントリーDOI: 10.2210/pdb8oi7/pdb
• 関連するPDBエントリー: 8OI9 8OIA 8OIB 8OIC
• 分子名称: Inosine-uridine preferring nucleoside hydrolase family protein, CALCIUM ION, DI(HYDROXYETHYL)ETHER, ... (6 entities in total)
• 機能のキーワード: nh-fold, nucleoside hydrolase, riboside hydrolase, nicotinamide riboside, hydrolase
• 由来する生物種: Trichomonas vaginalis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78919.77

構造登録者

Patrone, M.,Stockman, B.J.,Degano, M. (登録日: 2023-03-22, 公開日: 2023-08-02, 最終更新日: 2023-09-06)

主引用文献

Patrone, M.,Galasyn, G.S.,Kerin, F.,Nyitray, M.M.,Parkin, D.W.,Stockman, B.J.,Degano, M.
A riboside hydrolase that salvages both nucleobases and nicotinamide in the auxotrophic parasite Trichomonas vaginalis.
J.Biol.Chem., 299:105077-105077, 2023

PubMed Abstract: Pathogenic parasites of the Trichomonas genus are causative agents of sexually transmitted diseases affecting millions of individuals worldwide and whose outcome may include stillbirths and enhanced cancer risks and susceptibility to HIV infection. Trichomonas vaginalis relies on imported purine and pyrimidine nucleosides and nucleobases for survival, since it lacks the enzymatic activities necessary for de novo biosynthesis. Here we show that T. vaginalis additionally lacks homologues of the bacterial or mammalian enzymes required for the synthesis of the nicotinamide ring, a crucial component in the redox cofactors NAD and NADP. Moreover, we show that a yet fully uncharacterized T. vaginalis protein homologous to bacterial and protozoan nucleoside hydrolases is active as a pyrimidine nucleosidase but shows the highest specificity toward the NAD metabolite nicotinamide riboside. Crystal structures of the trichomonal riboside hydrolase in different states reveals novel intermediates along the nucleoside hydrolase-catalyzed hydrolytic reaction, including an unexpected asymmetry in the homotetrameric assembly. The active site structure explains the broad specificity toward different ribosides and offers precise insights for the engineering of specific inhibitors that may simultaneously target different essential pathways in the parasite.

PubMed: 37482279
DOI: 10.1016/j.jbc.2023.105077

実験手法

X-RAY DIFFRACTION (1.85 Å)