8KFO

Crystal structure of BSA in complex with B3

8KFO の概要

• エントリーDOI: 10.2210/pdb8kfo/pdb
• 分子名称: Albumin, 6-[(~{E})-2-[1-[2-[2-(2-methoxyethoxy)ethoxy]ethyl]pyridin-1-ium-4-yl]ethenyl]-~{N},~{N}-dimethyl-naphthalen-2-amine (2 entities in total)
• 機能のキーワード: albumin dimer, photo-sensitizer, transport protein
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 140900.56

構造登録者

Chen, X.,Ge, Y.H.,Yang, H.,Fang, B.,Li, L. (登録日: 2023-08-16, 公開日: 2024-08-21, 最終更新日: 2025-03-05)

主引用文献

Fang, B.,Bai, H.,Zhang, J.,Wang, L.,Li, P.,Ge, Y.,Yang, H.,Wang, H.,Peng, B.,Hu, W.,Ma, H.,Chen, X.,Fu, L.,Li, L.
Albumins constrainting the conformation of mitochondria-targeted photosensitizers for tumor-specific photodynamic therapy.
Biomaterials, 315:122914-122914, 2025

PubMed Abstract: Tumor ablation Preclinical organelle-targeted phototherapies have effectively achieved tumor photoablation for regenerative biomedical applications in cancer therapies. However, engineering effective phototherapy drugs with precise tumor-localization targeting and organelle direction remains challenging. Herein, we report a albumins constrainting mitochondrial-targeted photosensitizer nanoparticles (PSs@BSAs) for tumor-specific photodynamic therapy. X-ray crystallography elucidates the two-stage assembly mechanism of PSs@BSAs. Femtosecond transient absorption spectroscopy and quantum mechanical calculations reveal the implications of conformational dynamics at the excited state. PSs@BSAs can efficiently disable mitochondrial activity, and further disrupt tumor angiogenesis based on the photodynamic effect. This triggers a metabolic and oxidative stress crisis to facilitate photoablation of solid tumor and antitumor metastasis. The study fully elucidates the interdisciplinary issues of chemistry, physics, and biological interfaces, thereby opening new horizons to inspire the engineering of organelle-targeted tumor-specific photosensitizers for biomedical applications.

PubMed: 39461059
DOI: 10.1016/j.biomaterials.2024.122914

実験手法

X-RAY DIFFRACTION (3.6 Å)