8KB9

Structure of the WDR91 WD40 domain

8KB9 の概要

• エントリーDOI: 10.2210/pdb8kb9/pdb
• 分子名称: WD repeat-containing protein 91 (2 entities in total)
• 機能のキーワード: effector, gtpase, wd40, endocytosis
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39824.16

構造登録者

Li, J.,Ma, X.L.,Banerjee, S.,Dong, Z.G. (登録日: 2023-08-04, 公開日: 2024-08-07, 最終更新日: 2024-12-18)

主引用文献

Ma, X.,Li, J.,Liu, N.,Banerjee, S.,Hu, X.,Wang, X.,Dong, J.,Liu, K.,Yang, C.,Dong, Z.
Insights into the distinct membrane targeting mechanisms of WDR91 family proteins.
Structure, 32:2287-2300.e4, 2024

PubMed Abstract: WDR91 and SORF1, members of the WD repeat-containing protein 91 family, control phosphoinositide conversion by inhibiting phosphatidylinositol 3-kinase activity on endosomes, which promotes endosome maturation. Here, we report the crystal structure of the human WDR91 WD40 domain complexed with Rab7 that has an unusual interface at the C-terminus of the Rab7 switch II region. WDR91 is highly selective for Rab7 among the tested GTPases. A LIS1 homology (LisH) motif within the WDR91 N-terminal domain (NTD) mediates self-association and may contribute partly to the augmented interaction between full-length WDR91 and Rab7. Both the Rab7 binding site and the LisH motif are indispensable for WDR91 function in endocytic trafficking. For the WDR91 orthologue SORF1 lacking the C-terminal WD40 domain, a C-terminal amphipathic helix (AH) mediates strong interactions with liposomes containing acidic lipids. During evolution the human WDR91 ancestor gene might have acquired a WD40 domain to replace the AH for endosomal membrane targeting.

PubMed: 39426373
DOI: 10.1016/j.str.2024.09.023

実験手法

X-RAY DIFFRACTION (1.9 Å)