8K75

N-terminal domain of FIPV nucleocapsid protein

Summary for 8K75

• Entry DOI: 10.2210/pdb8k75/pdb
• Descriptor: Nucleoprotein (2 entities in total)
• Functional Keywords: fipv, nucleocapsid protein, viral protein
• Biological source: Feline coronavirus (strain FIPV WSU-79/1146) (FCoV)
• Total number of polymer chains: 1
• Total formula weight: 16618.93

Authors

Zhang, J.T.,Liang, R.,Shi, Y.J.,Peng, G.Q. (deposition date: 2023-07-26, release date: 2024-07-31, Last modification date: 2024-10-30)

Primary citation

Zhang, J.,Fan, X.,Wang, P.,Liang, R.,Wang, D.,Xu, J.,Zhang, D.,Xie, Y.,Liao, Q.,Jiao, Z.,Shi, Y.,Peng, G.
Identification of novel broad-spectrum antiviral drugs targeting the N-terminal domain of the FIPV nucleocapsid protein.
Int.J.Biol.Macromol., 279:135352-135352, 2024

PubMed Abstract: Coronaviruses pose serious threats to human and animal health worldwide, of which their structural nucleocapsid (N) proteins play multiple key roles in viral replication. However, the structures of animal coronavirus N proteins are poorly understood, posing challenges for research on their functions and pathogenic mechanisms as well as the development of N protein-based antiviral drugs. Therefore, N proteins must be further explored as potential antiviral targets. We determined the structure of the NNTD of feline infectious peritonitis virus (FIPV) and identified 3,6-dihydroxyflavone (3,6- DHF) as an effective N protein inhibitor. 3,6-DHF successfully inhibited FIPV replication in CRFK cells, showing broad-spectrum activity and effectiveness against drugresistant strains. Our study provides important insights for developing novel broadspectrum anti-coronavirus drugs and treating infections caused by drug-resistant mutant strains.

PubMed: 39242012
DOI: 10.1016/j.ijbiomac.2024.135352

Experimental method

X-RAY DIFFRACTION (1.16 Å)