8JTR

Cryo-EM structure of GeoCas9-sgRNA binary complex

Summary for 8JTR

Entry DOI	10.2210/pdb8jtr/pdb
EMDB information	36650
Descriptor	CRISPR-associated endonuclease Cas9, sgRNA (139-bp) (2 entities in total)
Functional Keywords	rna-binding, complex, gene editing, crispr-cas system, rna binding protein-rna complex, rna binding protein/rna
Biological source	Geobacillus stearothermophilus More
Total number of polymer chains	2
Total formula weight	172868.43
Authors	Shen, P.P.,Liu, B.B.,Li, X.,Zhang, L.L.,Chen, C.-C.,Guo, R.-T. (deposition date: 2023-06-22, release date: 2024-06-05, Last modification date: 2024-12-18)
Primary citation	Shen, P.,Zhang, L.,Liu, B.,Li, X.,Wang, C.,Min, J.,Huang, J.W.,Chen, C.C.,Guo, R.T. Structure of Geobacillus stearothermophilus Cas9: Insights into the Catalytic Process and Thermostability of CRISPR-Cas9 Acs Catalysis, 14:13227-13235, 2024 Cited by DOI: 10.1021/acscatal.4c03278 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.21 Å)

Structure validation