8JTK

Structure of AYWB phytoplasma SAP05 recognizing AtRpn10

Summary for 8JTK

• Entry DOI: 10.2210/pdb8jtk/pdb
• Descriptor: 26S proteasome non-ATPase regulatory subunit 4 homolog, Sequence-variable mosaic (SVM) signal sequence domain-containing protein (3 entities in total)
• Functional Keywords: complex, proteasome, hijack, plant protein
• Biological source: Arabidopsis thaliana (thale cress); More
• Total number of polymer chains: 2
• Total formula weight: 33190.75

Authors

Du, Y.X.,Zhang, L.Y.,Zheng, Q.Y. (deposition date: 2023-06-22, release date: 2023-07-19, Last modification date: 2024-07-17)

Primary citation

Zhang, L.,Du, Y.,Qu, Q.,Zheng, Q.
Structure basis for recognition of plant Rpn10 by phytoplasma SAP05 in ubiquitin-independent protein degradation.
Iscience, 27:108892-108892, 2024

PubMed Abstract: Besides traditional ubiquitin-dependent proteasome degradation, thousands of eukaryotic proteins more than previously appreciated could undergo ubiquitin-independent proteasomal degradation (UbInPD). A pathogen-encoded effector protein SAP05 secreted by phytoplasma, could hijack hostage Rpn10 subunit of proteasome derived from and target the degradation of GATA BINDING FACTOR (GATA) or SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors (TFs) without ubiquitin or additional proteasome shuttle factors. To explain how could SAP05 target the degradation bypassing the ubiquitin-dependent pathway, we have determined the crystal structure of the complex between Rpn10 and SAP05 or SAP05, which showed a previously unknown recognition interface. Sequence alignment and structural biological evidence showed that this interaction is highly conserved in various SAP05 homologs, suggesting a general mode in plant infection. After docking the complex structure to the plant proteasome, SAP05 was near to the adenosine triphosphatase (ATPase) central pore and enough to submit substrate to degradation process, which suggested a molecular glue-like role to bridge TFs close to the ATPase central pore of proteasomes for the direct degradation.

PubMed: 38322988
DOI: 10.1016/j.isci.2024.108892

Experimental method

X-RAY DIFFRACTION (1.57 Å)