8JP9

Cryo-EM structure of the head region of full-length ERGIC-53 with MCFD2 (Substate D)

8JP9 の概要

• エントリーDOI: 10.2210/pdb8jp9/pdb
• EMDBエントリー: 36472
• 分子名称: Protein ERGIC-53, Multiple coagulation factor deficiency protein 2, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: cargo receptor, calcium, zinc, protein transport
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 291775.87

構造登録者

Watanabe, S.,Inaba, K. (登録日: 2023-06-10, 公開日: 2024-04-17, 最終更新日: 2024-10-23)

主引用文献

Watanabe, S.,Kise, Y.,Yonezawa, K.,Inoue, M.,Shimizu, N.,Nureki, O.,Inaba, K.
Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport.
Nat Commun, 15:2404-2404, 2024

PubMed Abstract: ERGIC-53 transports certain subsets of newly synthesized secretory proteins and membrane proteins from the endoplasmic reticulum to the Golgi apparatus. Despite numerous structural and functional studies since its identification, the overall architecture and mechanism of action of ERGIC-53 remain unclear. Here we present cryo-EM structures of full-length ERGIC-53 in complex with its functional partner MCFD2. These structures reveal that ERGIC-53 exists as a homotetramer, not a homohexamer as previously suggested, and comprises a four-leaf clover-like head and a long stalk composed of three sets of four-helix coiled-coil followed by a transmembrane domain. 3D variability analysis visualizes the flexible motion of the long stalk and local plasticity of the head region. Notably, MCFD2 is shown to possess a Zn-binding site in its N-terminal lid, which appears to modulate cargo binding. Altogether, distinct mechanisms of cargo capture and release by ERGIC- 53 via the stalk bending and metal binding are proposed.

PubMed: 38493152
DOI: 10.1038/s41467-024-46747-1

実験手法

ELECTRON MICROSCOPY (3.37 Å)