8JO0

The Cryo-EM structure of a heptameric CED-4/CED-3 catalytic complex

8JO0 の概要

• エントリーDOI: 10.2210/pdb8jo0/pdb
• EMDBエントリー: 36451
• 分子名称: Cell death protein 4, MAGNESIUM ION, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: ced-4, ced-3 catalytic domain, apoptosis
• 由来する生物種: Caenorhabditis elegans; 詳細
• タンパク質・核酸の鎖数: 13
• 化学式量合計: 521995.13

構造登録者

Li, Y.,Shi, Y. (登録日: 2023-06-06, 公開日: 2023-06-28, 最終更新日: 2024-07-03)

主引用文献

Li, Y.,Tian, L.,Zhang, Y.,Shi, Y.
Structural insights into CED-3 activation.
Life Sci Alliance, 6:-, 2023

PubMed Abstract: In , onset of programmed cell death is marked with the activation of CED-3, a process that requires assembly of the CED-4 apoptosome. Activated CED-3 forms a holoenzyme with the CED-4 apoptosome to cleave a wide range of substrates, leading to irreversible cell death. Despite decades of investigations, the underlying mechanism of CED-4-facilitated CED-3 activation remains elusive. Here, we report cryo-EM structures of the CED-4 apoptosome and three distinct CED-4/CED-3 complexes that mimic different activation stages for CED-3. In addition to the previously reported octamer in crystal structures, CED-4, alone or in complex with CED-3, exists in multiple oligomeric states. Supported by biochemical analyses, we show that the conserved CARD-CARD interaction promotes CED-3 activation, and initiation of programmed cell death is regulated by the dynamic organization of the CED-4 apoptosome.

PubMed: 37402593
DOI: 10.26508/lsa.202302056

実験手法

ELECTRON MICROSCOPY (3.6 Å)