8JH2

RNA polymerase II elongation complex bound with Elf1, Spt4/5 and foreign DNA, stalled at SHL(-1) of the nucleosome

8JH2 の概要

• エントリーDOI: 10.2210/pdb8jh2/pdb
• EMDBエントリー: 36251
• 分子名称: DNA (40-MER), DNA-directed RNA polymerases I, II, and III subunit RPABC3, DNA-directed RNA polymerase subunit, ... (26 entities in total)
• 機能のキーワード: transcription-dna-rna complex, rnapii, cryo-em, transcription/dna/rna
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 917569.66

構造登録者

Akatsu, M.,Fujita, R.,Ogasawara, M.,Ehara, H.,Kujirai, T.,Takizawa, Y.,Sekine, S.,Kurumizaka, H. (登録日: 2023-05-22, 公開日: 2023-11-29, 最終更新日: 2023-12-20)

主引用文献

Akatsu, M.,Ehara, H.,Kujirai, T.,Fujita, R.,Ito, T.,Osumi, K.,Ogasawara, M.,Takizawa, Y.,Sekine, S.I.,Kurumizaka, H.
Cryo-EM structures of RNA polymerase II-nucleosome complexes rewrapping transcribed DNA.
J.Biol.Chem., 299:105477-105477, 2023

PubMed Abstract: RNA polymerase II (RNAPII) transcribes DNA wrapped in the nucleosome by stepwise pausing, especially at nucleosomal superhelical locations -5 and -1 [SHL(-5) and SHL(-1), respectively]. In the present study, we performed cryo-electron microscopy analyses of RNAPII-nucleosome complexes paused at a major nucleosomal pausing site, SHL(-1). We determined two previously undetected structures, in which the transcribed DNA behind RNAPII is sharply kinked at the RNAPII exit tunnel and rewrapped around the nucleosomal histones in front of RNAPII by DNA looping. This DNA kink shifts the DNA orientation toward the nucleosome, and the transcribed DNA region interacts with basic amino acid residues of histones H2A, H2B, and H3 exposed by the RNAPII-mediated nucleosomal DNA peeling. The DNA loop structure was not observed in the presence of the transcription elongation factors Spt4/5 and Elf1. These RNAPII-nucleosome structures provide important information for understanding the functional relevance of DNA looping during transcription elongation in the nucleosome.

PubMed: 37981206
DOI: 10.1016/j.jbc.2023.105477

実験手法

ELECTRON MICROSCOPY (5.7 Å)