8JF0

Human sodium-dependent vitamin C transporter 1 in an intermediate state

8JF0 の概要

• エントリーDOI: 10.2210/pdb8jf0/pdb
• 関連するPDBエントリー: 8JEW 8JEZ 8JF0
• EMDBエントリー: 36205
• 分子名称: Solute carrier family 23 member 1 (1 entity in total)
• 機能のキーワード: transporter, solute carrier, ascorbic acid, vitamin c, sodium, transport protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 64860.71

構造登録者

Kobayashi, T.A.,Kusakizako, T.,Nureki, O. (登録日: 2023-05-16, 公開日: 2024-05-22, 最終更新日: 2024-12-04)

主引用文献

Kobayashi, T.A.,Shimada, H.,Sano, F.K.,Itoh, Y.,Enoki, S.,Okada, Y.,Kusakizako, T.,Nureki, O.
Dimeric transport mechanism of human vitamin C transporter SVCT1.
Nat Commun, 15:5569-5569, 2024

PubMed Abstract: Vitamin C plays important roles as a cofactor in many enzymatic reactions and as an antioxidant against oxidative stress. As some mammals including humans cannot synthesize vitamin C de novo from glucose, its uptake from dietary sources is essential, and is mediated by the sodium-dependent vitamin C transporter 1 (SVCT1). Despite its physiological significance in maintaining vitamin C homeostasis, the structural basis of the substrate transport mechanism remained unclear. Here, we report the cryo-EM structures of human SVCT1 in different states at 2.5-3.5 Å resolutions. The binding manner of vitamin C together with two sodium ions reveals the counter ion-dependent substrate recognition mechanism. Furthermore, comparisons of the inward-open and occluded structures support a transport mechanism combining elevator and distinct rotational motions. Our results demonstrate the molecular mechanism of vitamin C transport with its underlying conformational cycle, potentially leading to future industrial and medical applications.

PubMed: 38956111
DOI: 10.1038/s41467-024-49899-2

実験手法

ELECTRON MICROSCOPY (3.5 Å)