8JAH

Crystal structure of human CLEC12A C-type lectin domain

8JAH の概要

• エントリーDOI: 10.2210/pdb8jah/pdb
• 分子名称: C-type lectin domain family 12 member A, SULFATE ION, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: receptor, structural protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29226.24

構造登録者

Lei, Q.,Tang, H.,Dong, X. (登録日: 2023-05-06, 公開日: 2024-03-13, 最終更新日: 2024-04-03)

主引用文献

Tang, H.,Xiao, Y.,Qian, L.,Wang, Z.,Lu, M.,Yao, N.,Zhou, T.,Tian, F.,Cao, L.,Zheng, P.,Dong, X.
Mechanistic insights into the C-type lectin receptor CLEC12A-mediated immune recognition of monosodium urate crystal.
J.Biol.Chem., 300:105765-105765, 2024

PubMed Abstract: CLEC12A, a member of the C-type lectin receptor family involved in immune homeostasis, recognizes MSU crystals released from dying cells. However, the molecular mechanism underlying the CLEC12A-mediated recognition of MSU crystals remains unclear. Herein, we reported the crystal structure of the human CLEC12A-C-type lectin-like domain (CTLD) and identified a unique "basic patch" site on CLEC12A-CTLD that is necessary for the binding of MSU crystals. Meanwhile, we determined the interaction strength between CLEC12A-CTLD and MSU crystals using single-molecule force spectroscopy. Furthermore, we found that CLEC12A clusters at the cell membrane and seems to serve as an internalizing receptor of MSU crystals. Altogether, these findings provide mechanistic insights for understanding the molecular mechanisms underlying the interplay between CLEC12A and MSU crystals.

PubMed: 38367667
DOI: 10.1016/j.jbc.2024.105765

実験手法

X-RAY DIFFRACTION (2.58 Å)