8J9Z

cryo-EM structure of viral topoisomerase in conformation 2

Summary for 8J9Z

• Entry DOI: 10.2210/pdb8j9z/pdb
• EMDB information: 36120
• Descriptor: DNA topoisomerase 2 (1 entity in total)
• Functional Keywords: topoisomerase, viral protein
• Biological source: African swine fever virus LIS57
• Total number of polymer chains: 2
• Total formula weight: 271375.72

Authors

Deng, Z. (deposition date: 2023-05-05, release date: 2023-08-16, Last modification date: 2023-11-29)

Primary citation

Zhao, Y.,Kuang, W.,An, Q.,Li, J.,Wang, Y.,Deng, Z.
Cryo-EM structures of African swine fever virus topoisomerase.
Mbio, 14:e0122823-e0122823, 2023

PubMed Abstract: African swine fever virus (ASFV) is a highly contagious virus that causes lethal hemorrhagic diseases known as African swine fever (ASF) with a case fatality rate of 100%. There is an urgent need to develop anti-ASFV drugs. We determine the first high-resolution structures of viral topoisomerase ASFV P1192R in both the closed and open C-gate forms. P1192R shows a similar overall architecture with eukaryotic and prokaryotic type II topoisomerases, which have been successful targets of many antimicrobials and anticancer drugs, with the most similarity to yeast topo II. P1192R also exhibits differences in the details of active site configuration, which are important to enzyme activity. These two structures offer useful structural information for antiviral drug design and provide structural evidence to support that eukaryotic type IIA topoisomerase likely originated from horizontal gene transfer from the virus.

PubMed: 37610250
DOI: 10.1128/mbio.01228-23

Experimental method

ELECTRON MICROSCOPY (3.13 Å)