8J9Q

Crystal structure of UBR box of UBR4 apo

8J9Q の概要

• エントリーDOI: 10.2210/pdb8j9q/pdb
• 分子名称: E3 ubiquitin-protein ligase UBR4, ZINC ION (3 entities in total)
• 機能のキーワード: e3 ligase, ubr box, ligase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 25304.58

構造登録者

Jeong, D.-E.,KIm, S.-J.,Shin, H.-C. (登録日: 2023-05-04, 公開日: 2023-12-06, 最終更新日: 2023-12-13)

主引用文献

Jeong, D.E.,Lee, H.S.,Ku, B.,Kim, C.H.,Kim, S.J.,Shin, H.C.
Insights into the recognition mechanism in the UBR box of UBR4 for its specific substrates.
Commun Biol, 6:1214-1214, 2023

PubMed Abstract: The N-end rule pathway is a proteolytic system involving the destabilization of N-terminal amino acids, known as N-degrons, which are recognized by N-recognins. Dysregulation of the N-end rule pathway results in the accumulation of undesired proteins, causing various diseases. The E3 ligases of the UBR subfamily recognize and degrade N-degrons through the ubiquitin-proteasome system. Herein, we investigated UBR4, which has a distinct mechanism for recognizing type-2 N-degrons. Structural analysis revealed that the UBR box of UBR4 differs from other UBR boxes in the N-degron binding sites. It recognizes type-2 N-terminal amino acids containing an aromatic ring and type-1 N-terminal arginine through two phenylalanines on its hydrophobic surface. We also characterized the binding mechanism for the second ligand residue. This is the report on the structural basis underlying the recognition of type-2 N-degrons by the UBR box with implications for understanding the N-end rule pathway.

PubMed: 38030679
DOI: 10.1038/s42003-023-05602-7

実験手法

X-RAY DIFFRACTION (2.18 Å)