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8J9P

SPARTA dimer bound with guide-target

8J9P の概要
エントリーDOI10.2210/pdb8j9p/pdb
EMDBエントリー36114
分子名称Piwi domain-containing protein, TIR domain-containing protein, RNA (5'-R(P*UP*GP*AP*CP*GP*GP*CP*UP*CP*UP*AP*AP*UP*CP*UP*AP*UP*UP*AP*GP*U)-3'), ... (5 entities in total)
機能のキーワードsparta, ago, tir, dna binding protein/rna/dna, dna binding protein-rna-dna complex
由来する生物種Thermoflavifilum thermophilum
詳細
タンパク質・核酸の鎖数8
化学式量合計251825.67
構造登録者
Li, Z.X.,Guo, L.J.,Huang, P.P.,Xiao, Y.B.,Chen, M.R. (登録日: 2023-05-04, 公開日: 2024-03-06, 最終更新日: 2024-04-10)
主引用文献Guo, L.,Huang, P.,Li, Z.,Shin, Y.C.,Yan, P.,Lu, M.,Chen, M.,Xiao, Y.
Auto-inhibition and activation of a short Argonaute-associated TIR-APAZ defense system.
Nat.Chem.Biol., 20:512-520, 2024
Cited by
PubMed Abstract: Short prokaryotic Ago accounts for most prokaryotic Argonaute proteins (pAgos) and is involved in defending bacteria against invading nucleic acids. Short pAgo associated with TIR-APAZ (SPARTA) has been shown to oligomerize and deplete NAD upon guide-mediated target DNA recognition. However, the molecular basis of SPARTA inhibition and activation remains unknown. In this study, we determined the cryogenic electron microscopy structures of Crenotalea thermophila SPARTA in its inhibited, transient and activated states. The SPARTA monomer is auto-inhibited by its acidic tail, which occupies the guide-target binding channel. Guide-mediated target binding expels this acidic tail and triggers substantial conformational changes to expose the Ago-Ago dimerization interface. As a result, SPARTA assembles into an active tetramer, where the four TIR domains are rearranged and packed to form NADase active sites. Together with biochemical evidence, our results provide a panoramic vision explaining SPARTA auto-inhibition and activation and expand understanding of pAgo-mediated bacterial defense systems.
PubMed: 37932527
DOI: 10.1038/s41589-023-01478-0
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (3.4 Å)
構造検証レポート
Validation report summary of 8j9p
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-10-30に公開中

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